Seafood as a Reservoir of Gram-negative Bacteria Carrying Integrons and Antimicrobial Resistance Genes in Japan

Ashraf M. Ahmed; Akito Maruyama; Hazim O. Khalifa; Toshi Shimamoto; Tadashi Shimamoto

doi:10.3967/bes2015.128

Seafood as a Reservoir of Gram-negative Bacteria Carrying Integrons and Antimicrobial Resistance Genes in Japan

Ashraf M. Ahmed
, Akito Maruyama
, Hazim O. Khalifa
, Toshi Shimamoto
, Tadashi Shimamoto

Research output: Contribution to journal › Letter › peer-review

25 Citations (Scopus)

Abstract

PCR and DNA sequencing were used to screen and characterize integrons and resistance genes in Gram-negative bacteria isolated from seafood products in Japan. A total of 215 Gram-negative bacteria were isolated from local and imported seafood samples collected from retail markets in Hiroshima Prefecture. Class 1 integrons containing gene cassettes encoding resistance to trimethoprim (dfrA12 and dfrA17), aminoglycosides (aadA2), and β-lactams (bla_PSE-1) were identified in six bacterial isolates. Four different β-lactamase-encoding genes including bla_TEM-1, bla_CMY-2, bla_CMY-13, and bla_CMY-39 were identified in seven isolates. A novel gene bla_CMY-39 was isolated from a strain of Citrobacter freundii. Plasmid-mediated quinolone resistance genes, including qnrB2, qnrB6, and qnrS1, were also identified in 10 isolates. This study highlights the presence of antimicrobial resistance genes in seafood-associated bacteria in Japan and indicates that seafood could be a reservoir and route of transmission of antibiotic-resistant bacteria to humans.

Original language	English
Pages (from-to)	924-926
Number of pages	3
Journal	Biomedical and Environmental Sciences
Volume	28
Issue number	12
DOIs	https://doi.org/10.3967/bes2015.128
Publication status	Published - Dec 1 2015
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis

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10.3967/bes2015.128

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title = "Seafood as a Reservoir of Gram-negative Bacteria Carrying Integrons and Antimicrobial Resistance Genes in Japan",

abstract = "PCR and DNA sequencing were used to screen and characterize integrons and resistance genes in Gram-negative bacteria isolated from seafood products in Japan. A total of 215 Gram-negative bacteria were isolated from local and imported seafood samples collected from retail markets in Hiroshima Prefecture. Class 1 integrons containing gene cassettes encoding resistance to trimethoprim (dfrA12 and dfrA17), aminoglycosides (aadA2), and β-lactams (blaPSE-1) were identified in six bacterial isolates. Four different β-lactamase-encoding genes including blaTEM-1, blaCMY-2, blaCMY-13, and blaCMY-39 were identified in seven isolates. A novel gene blaCMY-39 was isolated from a strain of Citrobacter freundii. Plasmid-mediated quinolone resistance genes, including qnrB2, qnrB6, and qnrS1, were also identified in 10 isolates. This study highlights the presence of antimicrobial resistance genes in seafood-associated bacteria in Japan and indicates that seafood could be a reservoir and route of transmission of antibiotic-resistant bacteria to humans.",

author = "Ahmed, \{Ashraf M.\} and Akito Maruyama and Khalifa, \{Hazim O.\} and Toshi Shimamoto and Tadashi Shimamoto",

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AU - Ahmed, Ashraf M.

AU - Maruyama, Akito

AU - Khalifa, Hazim O.

AU - Shimamoto, Toshi

AU - Shimamoto, Tadashi

PY - 2015/12/1

Y1 - 2015/12/1

N2 - PCR and DNA sequencing were used to screen and characterize integrons and resistance genes in Gram-negative bacteria isolated from seafood products in Japan. A total of 215 Gram-negative bacteria were isolated from local and imported seafood samples collected from retail markets in Hiroshima Prefecture. Class 1 integrons containing gene cassettes encoding resistance to trimethoprim (dfrA12 and dfrA17), aminoglycosides (aadA2), and β-lactams (blaPSE-1) were identified in six bacterial isolates. Four different β-lactamase-encoding genes including blaTEM-1, blaCMY-2, blaCMY-13, and blaCMY-39 were identified in seven isolates. A novel gene blaCMY-39 was isolated from a strain of Citrobacter freundii. Plasmid-mediated quinolone resistance genes, including qnrB2, qnrB6, and qnrS1, were also identified in 10 isolates. This study highlights the presence of antimicrobial resistance genes in seafood-associated bacteria in Japan and indicates that seafood could be a reservoir and route of transmission of antibiotic-resistant bacteria to humans.

AB - PCR and DNA sequencing were used to screen and characterize integrons and resistance genes in Gram-negative bacteria isolated from seafood products in Japan. A total of 215 Gram-negative bacteria were isolated from local and imported seafood samples collected from retail markets in Hiroshima Prefecture. Class 1 integrons containing gene cassettes encoding resistance to trimethoprim (dfrA12 and dfrA17), aminoglycosides (aadA2), and β-lactams (blaPSE-1) were identified in six bacterial isolates. Four different β-lactamase-encoding genes including blaTEM-1, blaCMY-2, blaCMY-13, and blaCMY-39 were identified in seven isolates. A novel gene blaCMY-39 was isolated from a strain of Citrobacter freundii. Plasmid-mediated quinolone resistance genes, including qnrB2, qnrB6, and qnrS1, were also identified in 10 isolates. This study highlights the presence of antimicrobial resistance genes in seafood-associated bacteria in Japan and indicates that seafood could be a reservoir and route of transmission of antibiotic-resistant bacteria to humans.

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JO - Biomedical and Environmental Sciences

JF - Biomedical and Environmental Sciences

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Seafood as a Reservoir of Gram-negative Bacteria Carrying Integrons and Antimicrobial Resistance Genes in Japan

Abstract

UN SDGs

ASJC Scopus subject areas

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