TY - JOUR
T1 - Sequences intervening between the core packaging determinants are dispensable for maintaining the packaging potential and propagation of feline immunodeficiency virus transfer vector RNAs
AU - Mustafa, Farah
AU - Ghazawi, Akela
AU - Jayanth, Preethi
AU - Phillip, Pretty Susan
AU - Ali, Jahabar
AU - Rizvi, Tahir A.
PY - 2005/11
Y1 - 2005/11
N2 - The packaging determinants of feline immunodeficiency virus (FIV) consist of two discontinuous core regions, extending from R to ∼150 bp of the 5′ untranslated region and the first ∼100 bp of gag. However, the role of sequences intervening between the core regions in packaging has not been clear. A mutational analysis was conducted to determine whether the intervening sequences played a role in FIV RNA packaging, using an in vivo packaging assay complemented with semiquantitative reverse transcriptase PCR. Our analyses reveal that the intervening sequences are dispensable not only for vector RNA packaging but also for propagation, confirming the discontinuous nature of the FIV packaging signal.
AB - The packaging determinants of feline immunodeficiency virus (FIV) consist of two discontinuous core regions, extending from R to ∼150 bp of the 5′ untranslated region and the first ∼100 bp of gag. However, the role of sequences intervening between the core regions in packaging has not been clear. A mutational analysis was conducted to determine whether the intervening sequences played a role in FIV RNA packaging, using an in vivo packaging assay complemented with semiquantitative reverse transcriptase PCR. Our analyses reveal that the intervening sequences are dispensable not only for vector RNA packaging but also for propagation, confirming the discontinuous nature of the FIV packaging signal.
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U2 - 10.1128/JVI.79.21.13817-13821.2005
DO - 10.1128/JVI.79.21.13817-13821.2005
M3 - Article
C2 - 16227303
AN - SCOPUS:27144453978
SN - 0022-538X
VL - 79
SP - 13817
EP - 13821
JO - Journal of Virology
JF - Journal of Virology
IS - 21
ER -