Serotonin reduces potassium current in rutabaga and wild-type Drosophila neurons

Waleed B. Alshuaib; Mini V. Mathew; Mohamed Y. Hasan; Mohamed Abdel Monem Fahim

doi:10.1080/00207450390242787

Serotonin reduces potassium current in rutabaga and wild-type Drosophila neurons

Waleed B. Alshuaib, Mini V. Mathew, Mohamed Y. Hasan, Mohamed Abdel Monem Fahim

Department of Pharmacology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The Drosophila learning mutant rutabaga is defective in short-term memory and has a reduced intracellular cyclic adenosine monophosphate (cAMP) concentration. The delayed-rectifier potassium current (IK_DR) was measured from cultured (2 days) wild-type and rutabaga neurons. IK_DR was smaller in rutabaga neurons (382 ± 41 pA) than in wild-type neurons (542 ± 33 pA). IK_DR was measured from neurons before and after addition of serotonin to the external solution. IK_DR was reduced by serotonin in wildtype (↓37 ± 7%) and rutabaga (↓33 ± 6%) neurons (single-cell studies). In the presence of serotonin, IK_DR was smaller in rutabaga (218 ± 24 pA) than in wild-type (426 ± 35 pA) neurons (population studies). These results indicate that serotonin has affected IK_DR so that the inherent difference between the two genotypes was preserved.

Original language	English
Pages (from-to)	1413-1425
Number of pages	13
Journal	International Journal of Neuroscience
Volume	113
Issue number	10
DOIs	https://doi.org/10.1080/00207450390242787
Publication status	Published - Oct 2003

Keywords

Learning mutants
Patch-clamp
Serotonin

ASJC Scopus subject areas

Neuroscience(all)

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10.1080/00207450390242787

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title = "Serotonin reduces potassium current in rutabaga and wild-type Drosophila neurons",

abstract = "The Drosophila learning mutant rutabaga is defective in short-term memory and has a reduced intracellular cyclic adenosine monophosphate (cAMP) concentration. The delayed-rectifier potassium current (IKDR) was measured from cultured (2 days) wild-type and rutabaga neurons. IKDR was smaller in rutabaga neurons (382 ± 41 pA) than in wild-type neurons (542 ± 33 pA). IKDR was measured from neurons before and after addition of serotonin to the external solution. IKDR was reduced by serotonin in wildtype (↓37 ± 7%) and rutabaga (↓33 ± 6%) neurons (single-cell studies). In the presence of serotonin, IKDR was smaller in rutabaga (218 ± 24 pA) than in wild-type (426 ± 35 pA) neurons (population studies). These results indicate that serotonin has affected IKDR so that the inherent difference between the two genotypes was preserved.",

keywords = "Learning mutants, Patch-clamp, Serotonin",

author = "Alshuaib, {Waleed B.} and Mathew, {Mini V.} and Hasan, {Mohamed Y.} and Fahim, {Mohamed Abdel Monem}",

note = "Funding Information: Received 10 December 2002. We thank Dr. Yi Zhong for providing mutant stocks. This work was supported by Kuwait University grant MPY032. Address correspondence to Dr. Waleed B. Alshuaib, Department of Physiology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait. E-mail: waleed@hsc.kuniv. edu.kw",

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AU - Hasan, Mohamed Y.

AU - Fahim, Mohamed Abdel Monem

N1 - Funding Information: Received 10 December 2002. We thank Dr. Yi Zhong for providing mutant stocks. This work was supported by Kuwait University grant MPY032. Address correspondence to Dr. Waleed B. Alshuaib, Department of Physiology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait. E-mail: waleed@hsc.kuniv. edu.kw

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N2 - The Drosophila learning mutant rutabaga is defective in short-term memory and has a reduced intracellular cyclic adenosine monophosphate (cAMP) concentration. The delayed-rectifier potassium current (IKDR) was measured from cultured (2 days) wild-type and rutabaga neurons. IKDR was smaller in rutabaga neurons (382 ± 41 pA) than in wild-type neurons (542 ± 33 pA). IKDR was measured from neurons before and after addition of serotonin to the external solution. IKDR was reduced by serotonin in wildtype (↓37 ± 7%) and rutabaga (↓33 ± 6%) neurons (single-cell studies). In the presence of serotonin, IKDR was smaller in rutabaga (218 ± 24 pA) than in wild-type (426 ± 35 pA) neurons (population studies). These results indicate that serotonin has affected IKDR so that the inherent difference between the two genotypes was preserved.

AB - The Drosophila learning mutant rutabaga is defective in short-term memory and has a reduced intracellular cyclic adenosine monophosphate (cAMP) concentration. The delayed-rectifier potassium current (IKDR) was measured from cultured (2 days) wild-type and rutabaga neurons. IKDR was smaller in rutabaga neurons (382 ± 41 pA) than in wild-type neurons (542 ± 33 pA). IKDR was measured from neurons before and after addition of serotonin to the external solution. IKDR was reduced by serotonin in wildtype (↓37 ± 7%) and rutabaga (↓33 ± 6%) neurons (single-cell studies). In the presence of serotonin, IKDR was smaller in rutabaga (218 ± 24 pA) than in wild-type (426 ± 35 pA) neurons (population studies). These results indicate that serotonin has affected IKDR so that the inherent difference between the two genotypes was preserved.

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Serotonin reduces potassium current in rutabaga and wild-type Drosophila neurons

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