Serotyping & molecular characterization for study of genetic diversity among seafood associated nontyphoidal Salmonella serovars

Patit Paban Bhowmick, Shabarinath Srikumar, Devananda Devegowda, Malathi Shekar, H. A. Darshanee Ruwandeepika, Indrani Karunasagar

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Background & objectives: Infections due to seafood associated Salmonella serovars are great risk to public health. Different phenotypic characteristics have been used previously for epidemiological investigation of Salmonella. Beyond the phenotypic characterization, a reliable genetic level discriminatory method is required. Therefore, this study was attempted to use different phenotypic and molecular fingerprinting methods for investigation of genetic diversity among seafood associated nontyphoidal Salmonella serovars. Methods: Fifty eight seafood associated Salmonella isolates were included in this study. All isolates were serotyped and epidemiological investigation was carried out using molecular fingerprinting methods, random amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus sequence based-PCR (ERIC-PCR) along with whole cell protein profiling using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) in our study. Results: Among the 58 Salmonella isolates, S. Weltevreden was observed to be the most predominant serovar. Typing of Salmonella serovars using RAPD and ERIC-PCR suggested the existence of a genetic diversity. Though both PCR based techniques were found to have a good discriminatory index, a better discriminatory ability was observed when the results obtained by the two techniques were combined and taken for composite analysis. Protein profiling of whole cells using SDS-PAGE demonstrated the presence of several bands with two bands of sizes 38 kDa and 46 kDa common among all 58 isolates. Interpretation & conclusions: Our study shows that use of protein profiling in combination with established typing methods such as RAPD and ERIC-PCR may provide useful information in typing of non-typhoidal Salmonella isolates associated with seafood and to develop strategies to protect public from Salmonella infections.

Original languageEnglish
Pages (from-to)371-381
Number of pages11
JournalIndian Journal of Medical Research
Volume135
Issue number3
Publication statusPublished - Mar 2012
Externally publishedYes

Keywords

  • ERIC
  • PCR
  • RAPD
  • Salmonella spp.
  • SDS-PAGE
  • Seafood
  • Serotype

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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