TY - JOUR
T1 - Significance of gastric endocrine tumor and age-related gut peptide alterations in Mastomys
AU - Bilchik, Anton J.
AU - Nilsson, Ola
AU - Modlin, Irvin M.
AU - Zucker, Karl A.
AU - Adrian, Thomas E.
N1 - Funding Information:
This work was supportedin part by grants from the Veterans Administration ResearchS ectiona ndthe Yale UniversityD epartmenotf SurgeryR esearchF und.O.N. was supportebdy FogartyIn ternationaCle nter,N IH (1 FO5 TWO 04145-01)S, wedish MRC (B89-14R-8617B, 89-14F-8616-01T),o re Nilsson Foundationa, nd the Swedish Societyo f Medicine.
PY - 1990/2/4
Y1 - 1990/2/4
N2 - The Mastomys (Praomys natalensis) species are a unique natural model in which the bioactivity of gastric carcinoids may be studied. Several investigators have previously demonstrated that these tumors contain large amounts of histamine. In this study we investigated the presence of peptides associated with the neoplasm. The levels and location of gastrin, gastric inhibitory peptide (GIP), neurotensin, peptide YY (PYY), pancreatic polypeptide (PP), glucagon, bombesin, vasoactive intestinal peptide (VIP) and somatostatin (SRIF) were investigated by radioimmunoassay and immunocytochemistry. In addition the distribution of these peptides were evaluated in the gastrointestinal tract of young and old animals to investigate possible age-related changes. PYY and enteroglucagon (EG) were significantly (P < 0.001) elevated in both tumor tissue (676 ± 152, 551 ± 164 pmol/g) and plasma (620 ± 160, 500 ± 147 pmol/l) of tumor-bearing animals. Immunocytochemistry revealed PYY- and EG-like immunoreactivity in 20-30% of tumor cells. A significant decrease (P < 0.05) in bombesin was noted in older animals, but no changes in gastric tissue content of PYY or EG could be detected between young and old animals. Gastrin was not detected in tumors and there were no significant changes in tissue or plasma levels with age. Small bowel concentrations of VIP and PYY were higher in the older mastomys (P < 0.05). In contrast, colonic levels of bombesin, VIP, somatostatin and PYY were significantly lower (P < 0.05) in older mastomys compared with young. The age-related changes in several peptides may reflect an adaptive response to acid hypersecretion. The multihormonal character of these neoplasms suggests that these tumors develop from a pluripotential stem cell.
AB - The Mastomys (Praomys natalensis) species are a unique natural model in which the bioactivity of gastric carcinoids may be studied. Several investigators have previously demonstrated that these tumors contain large amounts of histamine. In this study we investigated the presence of peptides associated with the neoplasm. The levels and location of gastrin, gastric inhibitory peptide (GIP), neurotensin, peptide YY (PYY), pancreatic polypeptide (PP), glucagon, bombesin, vasoactive intestinal peptide (VIP) and somatostatin (SRIF) were investigated by radioimmunoassay and immunocytochemistry. In addition the distribution of these peptides were evaluated in the gastrointestinal tract of young and old animals to investigate possible age-related changes. PYY and enteroglucagon (EG) were significantly (P < 0.001) elevated in both tumor tissue (676 ± 152, 551 ± 164 pmol/g) and plasma (620 ± 160, 500 ± 147 pmol/l) of tumor-bearing animals. Immunocytochemistry revealed PYY- and EG-like immunoreactivity in 20-30% of tumor cells. A significant decrease (P < 0.05) in bombesin was noted in older animals, but no changes in gastric tissue content of PYY or EG could be detected between young and old animals. Gastrin was not detected in tumors and there were no significant changes in tissue or plasma levels with age. Small bowel concentrations of VIP and PYY were higher in the older mastomys (P < 0.05). In contrast, colonic levels of bombesin, VIP, somatostatin and PYY were significantly lower (P < 0.05) in older mastomys compared with young. The age-related changes in several peptides may reflect an adaptive response to acid hypersecretion. The multihormonal character of these neoplasms suggests that these tumors develop from a pluripotential stem cell.
KW - Age related changes
KW - Gastric carcinoma
KW - Gastrointestinal peptides
KW - Mastomys
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U2 - 10.1016/0167-0115(90)90039-Y
DO - 10.1016/0167-0115(90)90039-Y
M3 - Article
C2 - 2326498
AN - SCOPUS:0025014799
SN - 0167-0115
VL - 27
SP - 195
EP - 207
JO - Regulatory Peptides
JF - Regulatory Peptides
IS - 2
ER -