TY - JOUR
T1 - Simple liquid chromatography method for the rapid simultaneous determination of prednisolone and cortisol in plasma and urine using hydrophilic lipophilic balanced solid phase extraction cartridges
AU - AbuRuz, S.
AU - Millership, J.
AU - Heaney, L.
AU - McElnay, J.
PY - 2003/12/25
Y1 - 2003/12/25
N2 - This article describes the development and validation of a simple solid phase extraction (SPE) and HPLC method for the extraction and the specific determination of prednisolone and hydrocortisone (cortisol) in both plasma and urine using one washing step with Oasis® hydrophilic lipophilic balanced (HLB) cartridges (1ml/30mg, 30μm). Recoveries of prednisolone and cortisol from plasma and urine exceeded 82%. The limit of quantification (LOQ) in plasma and urine was 9.9 and 6.7ng/ml for cortisol, respectively, and 11.6 and 8.0ng/ ml for prednisolone, respectively. The intraday and interday precision (measured by CV%) for both prednisolone and cortisol in both plasma and urine was always less than 7%. The accuracy (measured by relative error %) for both prednisolone and cortisol in both plasma and urine was always less than 8%. The advantages of the developed method are the use of a one step washing SPE utilising HLB cartridges which do not suffer the drying out problems of conventional SPE cartridges and the time saving when compared with solvent extraction (SE), in addition to the simultaneous determination of prednisolone and cortisol in both plasma and urine.
AB - This article describes the development and validation of a simple solid phase extraction (SPE) and HPLC method for the extraction and the specific determination of prednisolone and hydrocortisone (cortisol) in both plasma and urine using one washing step with Oasis® hydrophilic lipophilic balanced (HLB) cartridges (1ml/30mg, 30μm). Recoveries of prednisolone and cortisol from plasma and urine exceeded 82%. The limit of quantification (LOQ) in plasma and urine was 9.9 and 6.7ng/ml for cortisol, respectively, and 11.6 and 8.0ng/ ml for prednisolone, respectively. The intraday and interday precision (measured by CV%) for both prednisolone and cortisol in both plasma and urine was always less than 7%. The accuracy (measured by relative error %) for both prednisolone and cortisol in both plasma and urine was always less than 8%. The advantages of the developed method are the use of a one step washing SPE utilising HLB cartridges which do not suffer the drying out problems of conventional SPE cartridges and the time saving when compared with solvent extraction (SE), in addition to the simultaneous determination of prednisolone and cortisol in both plasma and urine.
KW - Cortisol
KW - Prednisolone
KW - Steroids
UR - http://www.scopus.com/inward/record.url?scp=0345708404&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0345708404&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2003.09.044
DO - 10.1016/j.jchromb.2003.09.044
M3 - Article
C2 - 14643497
AN - SCOPUS:0345708404
SN - 1570-0232
VL - 798
SP - 193
EP - 201
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -