TY - JOUR
T1 - Simultaneous and rapid quantification of microalga biomolecule content using electrochemical impedance spectroscopy
AU - Al Ahmad, Mahmoud
AU - Raji, Shaima
AU - Mustafa, Farah
AU - Rizvi, Tahir A.
AU - Al-Zuhair, Sulaiman
N1 - Funding Information:
This work was financially supported by the UAE University (Fund No. 31N163).
Funding Information:
This work was financially supported by the UAE University (Fund No. 31N163).
Publisher Copyright:
© 2020 American Institute of Chemical Engineers
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Lipids, proteins, and carbohydrates are the major constituents found in microalga cells, in varying proportions, and these biomolecules find applications in different industries. During microalga cultivation, to efficiently manipulate, control, and optimize the productivity of a specific compound for a specific application, real-time monitoring of these three cell components is essential. In this study, a method using measurement of electrical capacitance was developed to simultaneously determine the lipid, protein, and carbohydrate content of microalga cells without the requirement for any pre-processing steps. The marine microalga Nannochloropsis oculata was cultivated under nitrogen starvation conditions to induce lipid accumulation over a period of 22 days. The correlation between the electrical capacitance of the microalga culture and the intracellular biomolecule content (determined by standard techniques) was investigated, enabling subsequent deduction of microalga intracellular content from electrical capacitance of the culture. The accuracy and precision of the technique were proven by validating an independent sample. The main advantage of the proposed technique is its capability of quantifying microalga composition within a few minutes, significantly faster than currently available conventional techniques.
AB - Lipids, proteins, and carbohydrates are the major constituents found in microalga cells, in varying proportions, and these biomolecules find applications in different industries. During microalga cultivation, to efficiently manipulate, control, and optimize the productivity of a specific compound for a specific application, real-time monitoring of these three cell components is essential. In this study, a method using measurement of electrical capacitance was developed to simultaneously determine the lipid, protein, and carbohydrate content of microalga cells without the requirement for any pre-processing steps. The marine microalga Nannochloropsis oculata was cultivated under nitrogen starvation conditions to induce lipid accumulation over a period of 22 days. The correlation between the electrical capacitance of the microalga culture and the intracellular biomolecule content (determined by standard techniques) was investigated, enabling subsequent deduction of microalga intracellular content from electrical capacitance of the culture. The accuracy and precision of the technique were proven by validating an independent sample. The main advantage of the proposed technique is its capability of quantifying microalga composition within a few minutes, significantly faster than currently available conventional techniques.
KW - biomolecules content
KW - capacitance
KW - electrical characterization
KW - microalgae Nannochloropsis oculata
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U2 - 10.1002/btpr.3037
DO - 10.1002/btpr.3037
M3 - Article
C2 - 32533601
AN - SCOPUS:85087211451
SN - 8756-7938
VL - 36
JO - Biotechnology Progress
JF - Biotechnology Progress
IS - 6
M1 - e3037
ER -