TY - JOUR
T1 - Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC
AU - Nawaz, M.
AU - Arayne, M.
AU - Sultana, N.
AU - Haider, A.
AU - Hisaindee, S.
PY - 2014
Y1 - 2014
N2 - A simple, rapid, and sensitive high-performance liquid chromatography method was developed and validated for the simultaneous determination and quantification of fusidic acid and steroids (prednisone, betamethasone valerate, hydrocortisone acetate, and dexamethasone sodium) from bulk drugs and human plasma. A RP-HPLC, operated at ambient temperature, was equipped with a UV detector for monitoring the effluents at 235 nm. The mobile phase consisted of methanol, acetonitrile, and 0.05 M phosphoric acid (10:60:30, v/v/v), and separation was achieved on a Medeterrane, C18 (5 μm, 12.5 × 0.46 mm) column at a flow rate of 1.7 mL min-1. Calibration curves were linear over concentration range 0.625-10 μg mL-1 with correlation coefficient (r2) greater than 0.9999. The coefficient of variation (CV) and relative error (RE) for intra- and interassay were <2% and <1%, respectively. Interference of other already administered common medicaments, such as aspirin, paracetamol, caffeine, nicotine, and other plasma components, were not found.
AB - A simple, rapid, and sensitive high-performance liquid chromatography method was developed and validated for the simultaneous determination and quantification of fusidic acid and steroids (prednisone, betamethasone valerate, hydrocortisone acetate, and dexamethasone sodium) from bulk drugs and human plasma. A RP-HPLC, operated at ambient temperature, was equipped with a UV detector for monitoring the effluents at 235 nm. The mobile phase consisted of methanol, acetonitrile, and 0.05 M phosphoric acid (10:60:30, v/v/v), and separation was achieved on a Medeterrane, C18 (5 μm, 12.5 × 0.46 mm) column at a flow rate of 1.7 mL min-1. Calibration curves were linear over concentration range 0.625-10 μg mL-1 with correlation coefficient (r2) greater than 0.9999. The coefficient of variation (CV) and relative error (RE) for intra- and interassay were <2% and <1%, respectively. Interference of other already administered common medicaments, such as aspirin, paracetamol, caffeine, nicotine, and other plasma components, were not found.
KW - HPLC
KW - fusidic acid
KW - steroids
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UR - http://www.scopus.com/inward/citedby.url?scp=84893632835&partnerID=8YFLogxK
U2 - 10.1556/AChrom.26.2014.1.6
DO - 10.1556/AChrom.26.2014.1.6
M3 - Article
AN - SCOPUS:84893632835
SN - 1233-2356
VL - 26
SP - 57
EP - 66
JO - Acta Chromatographica
JF - Acta Chromatographica
IS - 1
ER -