Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC

M. Nawaz, M. Arayne, N. Sultana, A. Haider, S. Hisaindee

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A simple, rapid, and sensitive high-performance liquid chromatography method was developed and validated for the simultaneous determination and quantification of fusidic acid and steroids (prednisone, betamethasone valerate, hydrocortisone acetate, and dexamethasone sodium) from bulk drugs and human plasma. A RP-HPLC, operated at ambient temperature, was equipped with a UV detector for monitoring the effluents at 235 nm. The mobile phase consisted of methanol, acetonitrile, and 0.05 M phosphoric acid (10:60:30, v/v/v), and separation was achieved on a Medeterrane, C18 (5 μm, 12.5 × 0.46 mm) column at a flow rate of 1.7 mL min-1. Calibration curves were linear over concentration range 0.625-10 μg mL-1 with correlation coefficient (r2) greater than 0.9999. The coefficient of variation (CV) and relative error (RE) for intra- and interassay were <2% and <1%, respectively. Interference of other already administered common medicaments, such as aspirin, paracetamol, caffeine, nicotine, and other plasma components, were not found.

Original languageEnglish
Pages (from-to)57-66
Number of pages10
JournalActa Chromatographica
Volume26
Issue number1
DOIs
Publication statusPublished - Feb 13 2014

Keywords

  • HPLC
  • fusidic acid
  • steroids

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint

Dive into the research topics of 'Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC'. Together they form a unique fingerprint.

Cite this