Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion

Ji Kang Fang; Subbuswamy K. Prabu; Naresh B. Sepuri; Haider Raza; Hindupur K. Anandatheerthavarada; Domenico Galati; Joseph Spear; Narayan G. Avadhani

doi:10.1016/j.febslet.2007.02.042

Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion

Ji Kang Fang, Subbuswamy K. Prabu, Naresh B. Sepuri, Haider Raza, Hindupur K. Anandatheerthavarada, Domenico Galati, Joseph Spear, Narayan G. Avadhani

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.

Original language	English
Pages (from-to)	1302-1310
Number of pages	9
Journal	FEBS Letters
Volume	581
Issue number	7
DOIs	https://doi.org/10.1016/j.febslet.2007.02.042
Publication status	Published - Apr 3 2007
Externally published	Yes

Keywords

Cytochrome c oxidase
Myocardial ischemia/reperfusion
Nano-LC-MS/MS analysis
Protein kinase A
Subunit phosphorylation

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.02.042

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@article{dc4a7e2ddbc04f6d9378a39a8ce11a61,

title = "Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion",

abstract = "We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.",

keywords = "Cytochrome c oxidase, Myocardial ischemia/reperfusion, Nano-LC-MS/MS analysis, Protein kinase A, Subunit phosphorylation",

author = "Fang, {Ji Kang} and Prabu, {Subbuswamy K.} and Sepuri, {Naresh B.} and Haider Raza and Anandatheerthavarada, {Hindupur K.} and Domenico Galati and Joseph Spear and Avadhani, {Narayan G.}",

note = "Funding Information: This research was supported by NIH Grant GM-49683. We are thankful to members of the Avadhani lab for helpful suggestions and discussions.",

year = "2007",

month = apr,

day = "3",

doi = "10.1016/j.febslet.2007.02.042",

language = "English",

volume = "581",

pages = "1302--1310",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "7",

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TY - JOUR

T1 - Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion

AU - Fang, Ji Kang

AU - Prabu, Subbuswamy K.

AU - Sepuri, Naresh B.

AU - Raza, Haider

AU - Anandatheerthavarada, Hindupur K.

AU - Galati, Domenico

AU - Spear, Joseph

AU - Avadhani, Narayan G.

N1 - Funding Information: This research was supported by NIH Grant GM-49683. We are thankful to members of the Avadhani lab for helpful suggestions and discussions.

PY - 2007/4/3

Y1 - 2007/4/3

N2 - We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.

AB - We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.

KW - Cytochrome c oxidase

KW - Myocardial ischemia/reperfusion

KW - Nano-LC-MS/MS analysis

KW - Protein kinase A

KW - Subunit phosphorylation

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UR - http://www.scopus.com/inward/citedby.url?scp=33947368267&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2007.02.042

DO - 10.1016/j.febslet.2007.02.042

M3 - Article

C2 - 17349628

AN - SCOPUS:33947368267

SN - 0014-5793

VL - 581

SP - 1302

EP - 1310

JO - FEBS Letters

JF - FEBS Letters

IS - 7

ER -

Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion

Abstract

Keywords

ASJC Scopus subject areas

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