TY - JOUR
T1 - Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion
AU - Fang, Ji Kang
AU - Prabu, Subbuswamy K.
AU - Sepuri, Naresh B.
AU - Raza, Haider
AU - Anandatheerthavarada, Hindupur K.
AU - Galati, Domenico
AU - Spear, Joseph
AU - Avadhani, Narayan G.
N1 - Funding Information:
This research was supported by NIH Grant GM-49683. We are thankful to members of the Avadhani lab for helpful suggestions and discussions.
PY - 2007/4/3
Y1 - 2007/4/3
N2 - We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.
AB - We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.
KW - Cytochrome c oxidase
KW - Myocardial ischemia/reperfusion
KW - Nano-LC-MS/MS analysis
KW - Protein kinase A
KW - Subunit phosphorylation
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U2 - 10.1016/j.febslet.2007.02.042
DO - 10.1016/j.febslet.2007.02.042
M3 - Article
C2 - 17349628
AN - SCOPUS:33947368267
SN - 0014-5793
VL - 581
SP - 1302
EP - 1310
JO - FEBS Letters
JF - FEBS Letters
IS - 7
ER -