Smooth muscle tissue engineering in crosslinked electrospun gelatin scaffolds

Yahya Elsayed, Constantina Lekakou, Fatima Labeed, Paul Tomlins

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Crosslinked, multi-layer electrospun gelatin fiber scaffolds with generally ±45 degree fiber orientation have been used to grow human umbilical vein smooth muscle cells (HUVSMCs) to create a vascular tunica media graft. Scaffolds of different fiber diameter (2-5 μm in wet state), pore size, and porosity (16-21% in wet state) were assessed in terms of cell adherence and viability, cell proliferation, and migration in both in-plane and transverse directions through the scaffold as a function of time under static cell culture conditions. HUVSMC cell viability reached between 80 and 92% for all scaffolds after 9 days in culture. HUVSMCs adhered, elongated, and orientated in the fiber direction, and migrated through a scaffold thickness of 200-235 μm 9 days post-seeding under static conditions. The best scaffold was then used to assess the tissue engineering of HUVSMCs under dynamic conditions for a rotating, cell seeded, tubular scaffold in the bioreactor containing the culture medium. Dynamic conditions almost doubled the rate of cell proliferation through the scaffold, forming full tissue throughout a scaffold of 250-300 μm thickness 6 days post-seeding.

Original languageEnglish
Pages (from-to)313-321
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume104
Issue number1
DOIs
Publication statusPublished - Jan 1 2016
Externally publishedYes

Keywords

  • cell migration
  • smooth muscle cells
  • tissue engineering
  • tunica media
  • vascular graft

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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