TY - JOUR
T1 - Somatic embryogenesis, scanning electron microscopy, histology and biochemical analysis at different developing stages of embryogenesis in six date palm (Phoenix dactylifera L.) cultivars
AU - Aslam, Junaid
AU - Khan, Saeed Ahmad
AU - Cheruth, Abdul Jaleel
AU - Mujib, Abdul
AU - Sharma, Maheshwar Pershad
AU - Srivastava, Prem Shanker
PY - 2011/10
Y1 - 2011/10
N2 - An efficient somatic embryogenesis system has been established in six date palm (Phoenix dactylifera L.) cultivars (Barhee, Zardai, Khalasah, Muzati, Shishi and Zart). Somatic embryogenesis (SE) was growth regulators and cultivars dependent. Friable embryogenic callus was induced from excised shoot tips on MS medium supplemented with various auxins particularly 2,4-dichlorophenoxyacetic acid (2,4-D, 1.5mg1-l). Suspension culture increased embryogenesis potentiality. Only a-naphthaleneacetic acid (NAA, 0.5mg1-1) produced somatic embryos in culture. Somatic embryos germinated and converted into plantlets in N6-benzyladenine (BAP, 0.75mg1-l) added medium following a treatment with thidiazuron (TDZ, 1.0mg1-l) for maturation. Scanning electron microscopy showed early stages of somatic embryo particularly, globular types, and was in masses. Different developing stages of embryogenesis (heart, torpedo and cotyledonary) were observed under histological preparation of embryogenic callus. Biochemical screening at various stages of somatic embryogenesis (embryogenic callus, somatic embryos, matured, germinated embryos and converted plantlets) of date palm cultivars has been conducted and discussed in detail. The result discussed in this paper indicates that somatic embryos were produced in numbers and converted plantlets can be used as a good source of alternative propagation. Genetic modification to the embryo precursor cell may improve the fruit quality and yield further.
AB - An efficient somatic embryogenesis system has been established in six date palm (Phoenix dactylifera L.) cultivars (Barhee, Zardai, Khalasah, Muzati, Shishi and Zart). Somatic embryogenesis (SE) was growth regulators and cultivars dependent. Friable embryogenic callus was induced from excised shoot tips on MS medium supplemented with various auxins particularly 2,4-dichlorophenoxyacetic acid (2,4-D, 1.5mg1-l). Suspension culture increased embryogenesis potentiality. Only a-naphthaleneacetic acid (NAA, 0.5mg1-1) produced somatic embryos in culture. Somatic embryos germinated and converted into plantlets in N6-benzyladenine (BAP, 0.75mg1-l) added medium following a treatment with thidiazuron (TDZ, 1.0mg1-l) for maturation. Scanning electron microscopy showed early stages of somatic embryo particularly, globular types, and was in masses. Different developing stages of embryogenesis (heart, torpedo and cotyledonary) were observed under histological preparation of embryogenic callus. Biochemical screening at various stages of somatic embryogenesis (embryogenic callus, somatic embryos, matured, germinated embryos and converted plantlets) of date palm cultivars has been conducted and discussed in detail. The result discussed in this paper indicates that somatic embryos were produced in numbers and converted plantlets can be used as a good source of alternative propagation. Genetic modification to the embryo precursor cell may improve the fruit quality and yield further.
KW - Amino acid
KW - Amino acids
KW - Date palm cultivars
KW - Histological analysis
KW - Protein
KW - Scanning electron microscopy
KW - Somatic embryogenesis
KW - Sugar
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U2 - 10.1016/j.sjbs.2011.06.002
DO - 10.1016/j.sjbs.2011.06.002
M3 - Article
C2 - 23961149
AN - SCOPUS:80052926079
SN - 1319-562X
VL - 18
SP - 369
EP - 380
JO - Saudi Journal of Biological Sciences
JF - Saudi Journal of Biological Sciences
IS - 4
ER -