TY - JOUR
T1 - Spoligotyping of mycobacterium tuberculosis isolates from tuberculosis diagnosed patients at dilla university referral hospital and other private clinics, Southern Ethiopia
AU - Gebrezgabiher, Gebremedhin
AU - Romha, Gebremedhin
AU - Ameni, Gobena
N1 - Funding Information:
This research was financially partially supported by Dilla University (Grant No. CAN/09/2012), and laboratory materials and reagents were provided by Aklilu Lemma Institute of Pathobiology (ALIPB), special TB laboratory, Addis Ababa University. The authors are thankful to Dr. Girma Ababi, a pathologist at Selam Higher Clinic, Mr. Asnake Dechasa, laboratory technician at Selam Higher Clinic, Sister Meseret Habtamu, TB expert and all the laboratory technicians working at Dilla University Referral Hospital, and TB experts of ALIPB for their unreserved help during data collection and processing. We are grateful to the TB patients who were willing to involve in this study. We also appreciate Dilla University Referral Hospital, Selam and Dr. Muluneh private higher clinics for allowing us to collect sputum and FNA samples.
Publisher Copyright:
© 2015 Asian Pacific Tropical Medicine Press.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Objective: To assess Mycobacterium tuberculosis (M. tuberculosis) strains exsisting in Gedeo zone and the surrounding areas of the Southern Ethiopia using spoligotyping. Methods: A cross sectional study was carried out from February, 2012 to June, 2013 and 97 (76 sputum and 21 fine needle aspirate) samples were taken from tuberculosis diagnosed patients at Dilla University Referral Hospital and other private clinics. Culturing, region of difference (RD9) deletion typing and spoligotyping techniques were employed to isolate M. tuberculosis strains. Results: Growth of mycobacteria was observed in 35.1% (34/97). Speciation of isolates showed that 91.2% (31/34) of the isolates were M. tuberculosis. Further characterization led to the identification of 23 different spoligotype patterns of M. tuberculosis of which 61% and 39% displayed unique and cluster patterns, respectively. The most dominant shared type was spoligotype international type 53. Of the 23 strains, 12 have not been registered in the international spoligotyping database (SpolDB4). Seventy one percent of the strains belonged to the Euro-American lineage. Conclusions: This study revealed the existence of both genetically diverse and clustered M. tuberculosis strains from tuberculosis patients in the area, suggesting reactivation of infection and recent transmission, respectively. Molecular epidemiology of M. tuberculosis should be done nationwide in order to set appropriate control measures.
AB - Objective: To assess Mycobacterium tuberculosis (M. tuberculosis) strains exsisting in Gedeo zone and the surrounding areas of the Southern Ethiopia using spoligotyping. Methods: A cross sectional study was carried out from February, 2012 to June, 2013 and 97 (76 sputum and 21 fine needle aspirate) samples were taken from tuberculosis diagnosed patients at Dilla University Referral Hospital and other private clinics. Culturing, region of difference (RD9) deletion typing and spoligotyping techniques were employed to isolate M. tuberculosis strains. Results: Growth of mycobacteria was observed in 35.1% (34/97). Speciation of isolates showed that 91.2% (31/34) of the isolates were M. tuberculosis. Further characterization led to the identification of 23 different spoligotype patterns of M. tuberculosis of which 61% and 39% displayed unique and cluster patterns, respectively. The most dominant shared type was spoligotype international type 53. Of the 23 strains, 12 have not been registered in the international spoligotyping database (SpolDB4). Seventy one percent of the strains belonged to the Euro-American lineage. Conclusions: This study revealed the existence of both genetically diverse and clustered M. tuberculosis strains from tuberculosis patients in the area, suggesting reactivation of infection and recent transmission, respectively. Molecular epidemiology of M. tuberculosis should be done nationwide in order to set appropriate control measures.
KW - Dilla University
KW - Mycobacterium tuberculosis strains
KW - Southern Ethiopia
KW - Spoligotyping
UR - http://www.scopus.com/inward/record.url?scp=84923201121&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84923201121&partnerID=8YFLogxK
U2 - 10.1016/S2222-1808(14)60792-1
DO - 10.1016/S2222-1808(14)60792-1
M3 - Article
AN - SCOPUS:84923201121
SN - 2222-1808
VL - 5
SP - 329
EP - 333
JO - Asian Pacific Journal of Tropical Disease
JF - Asian Pacific Journal of Tropical Disease
IS - 4
ER -