TY - JOUR
T1 - Step elution in preparative liquid chromatography
AU - Hu, S. G.
AU - Do, D. D.
AU - Hossain, Md M.
N1 - Funding Information:
M.M.H. acknowledgesth e support of the Australian NRF in the form of a fellowship.
PY - 1992/7/17
Y1 - 1992/7/17
N2 - Preparative liquid chromatography with the step elution, in which a sample is loaded into a column at higher binding strength and then eluted at lower binding strength, was numerically simulated with a detailed rate equation model. The model takes into account film mass transfer resistance, pore diffusion, axial dispersion and local equilibrium. The step elution mode was compared with the isocratic elution mode in terms of the preparative performance for a binary mixture having a constant or variable separation factor. The effects of various parameters, such as step height, sample size, solute concentration, feed composition and mobile phase flow-rate, were examined.
AB - Preparative liquid chromatography with the step elution, in which a sample is loaded into a column at higher binding strength and then eluted at lower binding strength, was numerically simulated with a detailed rate equation model. The model takes into account film mass transfer resistance, pore diffusion, axial dispersion and local equilibrium. The step elution mode was compared with the isocratic elution mode in terms of the preparative performance for a binary mixture having a constant or variable separation factor. The effects of various parameters, such as step height, sample size, solute concentration, feed composition and mobile phase flow-rate, were examined.
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U2 - 10.1016/0021-9673(92)85235-L
DO - 10.1016/0021-9673(92)85235-L
M3 - Article
AN - SCOPUS:0026773563
SN - 0021-9673
VL - 605
SP - 175
EP - 191
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -