The direct effects of streptozotocin and alloxan on contractile function in rat heart

K. A. Salem; M. Kosanovic; A. Qureshi; M. Ljubisavljevic; F. C. Howarth

doi:10.1016/j.phrs.2009.01.003

The direct effects of streptozotocin and alloxan on contractile function in rat heart

K. A. Salem, M. Kosanovic, A. Qureshi, M. Ljubisavljevic, F. C. Howarth

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Streptozotocin (STZ) and alloxan (ALX) are widely used to induce diabetes mellitus in experimental animals. The direct effects of STZ and ALX on the amplitude and time course of ventricular myocyte shortening and on cardiac action potentials were investigated. STZ and ALX (10^-5 M) were dissolved in normal Tyrode (NT), maintained at pH 7.4 and 37 °C and stored for either 15 or 60-120 min. Both compounds reduced the amplitude of myocyte shortening. Compared to NT the amplitude of shortening was 34.7 ± 5.0% and 35.2 ± 6.8% with STZ and 41.0 ± 5.5% and 37.3 ± 5.7% with ALX stored for 15 and 60-120 min, respectively. During a 10 min NT washout STZ myocytes recovered to 56.2 ± 8.3% and 60.5 ± 8.2% and ALX myocytes recovered to 88.9 ± 10.0% and 83.7 ± 9.9% after storage of compounds for 15 and 60-120 min, respectively. Perfusion of the whole heart with ALX induced bradycardia but had no effects on the duration of action potential repolarization at 50% and 70% from peak action potential. The negative inotropic effects of STZ and ALX were not altered by storage. The results suggest that some of the effects on heart reported in STZ- and ALX-induced diabetes may be partly attributed to direct action of these compounds.

Original language	English
Pages (from-to)	235-241
Number of pages	7
Journal	Pharmacological Research
Volume	59
Issue number	4
DOIs	https://doi.org/10.1016/j.phrs.2009.01.003
Publication status	Published - Apr 2009

Keywords

Action potential
Alloxan
Contraction
Diabetes
Myocyte
Streptozotocin
Ventricular myocytes

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/j.phrs.2009.01.003

Cite this

@article{5ca74b9e746f44a4a60619e114bf1a21,

title = "The direct effects of streptozotocin and alloxan on contractile function in rat heart",

abstract = "Streptozotocin (STZ) and alloxan (ALX) are widely used to induce diabetes mellitus in experimental animals. The direct effects of STZ and ALX on the amplitude and time course of ventricular myocyte shortening and on cardiac action potentials were investigated. STZ and ALX (10-5 M) were dissolved in normal Tyrode (NT), maintained at pH 7.4 and 37 °C and stored for either 15 or 60-120 min. Both compounds reduced the amplitude of myocyte shortening. Compared to NT the amplitude of shortening was 34.7 ± 5.0% and 35.2 ± 6.8% with STZ and 41.0 ± 5.5% and 37.3 ± 5.7% with ALX stored for 15 and 60-120 min, respectively. During a 10 min NT washout STZ myocytes recovered to 56.2 ± 8.3% and 60.5 ± 8.2% and ALX myocytes recovered to 88.9 ± 10.0% and 83.7 ± 9.9% after storage of compounds for 15 and 60-120 min, respectively. Perfusion of the whole heart with ALX induced bradycardia but had no effects on the duration of action potential repolarization at 50% and 70% from peak action potential. The negative inotropic effects of STZ and ALX were not altered by storage. The results suggest that some of the effects on heart reported in STZ- and ALX-induced diabetes may be partly attributed to direct action of these compounds.",

keywords = "Action potential, Alloxan, Contraction, Diabetes, Myocyte, Streptozotocin, Ventricular myocytes",

author = "Salem, {K. A.} and M. Kosanovic and A. Qureshi and M. Ljubisavljevic and Howarth, {F. C.}",

note = "Funding Information: This research was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Export Promotion Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA). (No. 316014-03) and by the Chung-Ang University Research Grant in 2018.",

year = "2009",

month = apr,

doi = "10.1016/j.phrs.2009.01.003",

language = "English",

volume = "59",

pages = "235--241",

journal = "Pharmacological Research",

issn = "1043-6618",

publisher = "Academic Press",

number = "4",

}

TY - JOUR

T1 - The direct effects of streptozotocin and alloxan on contractile function in rat heart

AU - Salem, K. A.

AU - Kosanovic, M.

AU - Qureshi, A.

AU - Ljubisavljevic, M.

AU - Howarth, F. C.

N1 - Funding Information: This research was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Export Promotion Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA). (No. 316014-03) and by the Chung-Ang University Research Grant in 2018.

PY - 2009/4

Y1 - 2009/4

N2 - Streptozotocin (STZ) and alloxan (ALX) are widely used to induce diabetes mellitus in experimental animals. The direct effects of STZ and ALX on the amplitude and time course of ventricular myocyte shortening and on cardiac action potentials were investigated. STZ and ALX (10-5 M) were dissolved in normal Tyrode (NT), maintained at pH 7.4 and 37 °C and stored for either 15 or 60-120 min. Both compounds reduced the amplitude of myocyte shortening. Compared to NT the amplitude of shortening was 34.7 ± 5.0% and 35.2 ± 6.8% with STZ and 41.0 ± 5.5% and 37.3 ± 5.7% with ALX stored for 15 and 60-120 min, respectively. During a 10 min NT washout STZ myocytes recovered to 56.2 ± 8.3% and 60.5 ± 8.2% and ALX myocytes recovered to 88.9 ± 10.0% and 83.7 ± 9.9% after storage of compounds for 15 and 60-120 min, respectively. Perfusion of the whole heart with ALX induced bradycardia but had no effects on the duration of action potential repolarization at 50% and 70% from peak action potential. The negative inotropic effects of STZ and ALX were not altered by storage. The results suggest that some of the effects on heart reported in STZ- and ALX-induced diabetes may be partly attributed to direct action of these compounds.

AB - Streptozotocin (STZ) and alloxan (ALX) are widely used to induce diabetes mellitus in experimental animals. The direct effects of STZ and ALX on the amplitude and time course of ventricular myocyte shortening and on cardiac action potentials were investigated. STZ and ALX (10-5 M) were dissolved in normal Tyrode (NT), maintained at pH 7.4 and 37 °C and stored for either 15 or 60-120 min. Both compounds reduced the amplitude of myocyte shortening. Compared to NT the amplitude of shortening was 34.7 ± 5.0% and 35.2 ± 6.8% with STZ and 41.0 ± 5.5% and 37.3 ± 5.7% with ALX stored for 15 and 60-120 min, respectively. During a 10 min NT washout STZ myocytes recovered to 56.2 ± 8.3% and 60.5 ± 8.2% and ALX myocytes recovered to 88.9 ± 10.0% and 83.7 ± 9.9% after storage of compounds for 15 and 60-120 min, respectively. Perfusion of the whole heart with ALX induced bradycardia but had no effects on the duration of action potential repolarization at 50% and 70% from peak action potential. The negative inotropic effects of STZ and ALX were not altered by storage. The results suggest that some of the effects on heart reported in STZ- and ALX-induced diabetes may be partly attributed to direct action of these compounds.

KW - Action potential

KW - Alloxan

KW - Contraction

KW - Diabetes

KW - Myocyte

KW - Streptozotocin

KW - Ventricular myocytes

UR - http://www.scopus.com/inward/record.url?scp=61349156674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61349156674&partnerID=8YFLogxK

U2 - 10.1016/j.phrs.2009.01.003

DO - 10.1016/j.phrs.2009.01.003

M3 - Article

C2 - 19429464

AN - SCOPUS:61349156674

SN - 1043-6618

VL - 59

SP - 235

EP - 241

JO - Pharmacological Research

JF - Pharmacological Research

IS - 4

ER -

The direct effects of streptozotocin and alloxan on contractile function in rat heart

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this