TY - JOUR
T1 - The Drosophila IgC2 domain protein friend-of-echinoid, a paralogue of echinoid, limits the number of sensory organ precursors in the wing disc and interacts with the Notch signaling pathway
AU - Chandra, Shweta
AU - Ahmed, Amina
AU - Vaessin, Harald
N1 - Funding Information:
We thank Drs. C. Delidakis, S. Bray, A. Garcia-Bellido, C. T. Chein, Y. N. Jan, J. Modolell, as well as the Bloomington Drosophila Stock Center for providing us with numerous fly stocks. We thank the Berkeley Drosophila Genome Project and the Anopheles Genome Project for excellent genomic sequence information. We thank Dr. S. Campuzano for helpful directions for staining with anti-dp-ERK antibody. We thank Drs. R. Cagan and S. Spencer for a creative name for fred . We also thank Drs. A. Simcox and J. Mustard for helpful comments on the manuscript. We also thank an anonymous reviewer for helping us to identify additional domains in Fred and Ed. DNA sequencing was performed at Neurobiotechnology Center DNA sequencing facility at The Ohio State University. The Campus Microscopy Imaging Facility at The Ohio State University was used for generating SEM. This work was supported by grants from the American Cancer Society and NSF (to H.V.).
PY - 2003/4/15
Y1 - 2003/4/15
N2 - The Notch signaling pathway is critical in cell fate specification throughout development. In the developing wing disc, single sensory organ precursors (SOPs) are selected from proneural clusters via a process of lateral inhibition mediated by the Notch signaling pathway. The epidermal growth factor receptor (EGFR) pathway has also been implicated in SOP formation. Here, we describe the Drosophila melanogaster gene friend of echinoid (fred), a paralogue of echinoid (ed), a gene recently identified as a negative regulator of the EGFR pathway. fred function was examined in transgenic flies by using inducible RNA interference (RNAi). Suppression of fred in developing wing discs results in specification of ectopic SOPs, additional microchaeta, and cell death. In eye-antennal discs, fred suppression causes a rough eye phenotype. These phenotypes are suppressed by overexpression of Notch, Suppressor of Hairless [Su(H)], and Enhancer of split m7. In contrast, overexpression of Hairless, a negative regulator of the Notch pathway, and decreased Su(H) activity enhance these phenotypes. Thus, fred acts in close concert with the Notch signaling pathway. Dosage-sensitive genetic interaction also suggests a close relationship between fred and ed.
AB - The Notch signaling pathway is critical in cell fate specification throughout development. In the developing wing disc, single sensory organ precursors (SOPs) are selected from proneural clusters via a process of lateral inhibition mediated by the Notch signaling pathway. The epidermal growth factor receptor (EGFR) pathway has also been implicated in SOP formation. Here, we describe the Drosophila melanogaster gene friend of echinoid (fred), a paralogue of echinoid (ed), a gene recently identified as a negative regulator of the EGFR pathway. fred function was examined in transgenic flies by using inducible RNA interference (RNAi). Suppression of fred in developing wing discs results in specification of ectopic SOPs, additional microchaeta, and cell death. In eye-antennal discs, fred suppression causes a rough eye phenotype. These phenotypes are suppressed by overexpression of Notch, Suppressor of Hairless [Su(H)], and Enhancer of split m7. In contrast, overexpression of Hairless, a negative regulator of the Notch pathway, and decreased Su(H) activity enhance these phenotypes. Thus, fred acts in close concert with the Notch signaling pathway. Dosage-sensitive genetic interaction also suggests a close relationship between fred and ed.
KW - Drosophila
KW - Echinoid
KW - Fred
KW - IgC2 domain
KW - Notch
KW - RNAi
KW - Sensory organ precursor
UR - http://www.scopus.com/inward/record.url?scp=0037446087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037446087&partnerID=8YFLogxK
U2 - 10.1016/S0012-1606(03)00038-1
DO - 10.1016/S0012-1606(03)00038-1
M3 - Article
C2 - 12679104
AN - SCOPUS:0037446087
SN - 0012-1606
VL - 256
SP - 302
EP - 316
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -