The effect of cerebral hypothermia on white and grey matter injury induced by severe hypoxia in preterm fetal sheep

L. Bennet, V. Roelfsema, S. George, J. M. Dean, B. S. Emerald, A. J. Gunn

Research output: Contribution to journalArticlepeer-review

115 Citations (Scopus)


Prolonged, moderate cerebral hypothermia is consistently neuroprotective after experimental hypoxia-ischaemia; however, it has not been tested in the preterm brain. Preterm (0.7 gestation) fetal sheep received complete umbilical cord occlusion for 25 min followed by cerebral hypothermia (fetal extradural temperature reduced from 39.4 ± 0.3 to 29.5 ± 2.6°C) from 90 min to 70 h after the end of occlusion or sham cooling. Occlusion led to severe acidosis and profound hypotension, which recovered rapidly after release of occlusion. After 3 days recovery the EEG spectral frequency, but not total intensity, was increased in the hypothermia-occlusion group compared with normothermia-occlusion. Hypothermia was associated with a significant overall reduction in loss of immature oligodendrocytes in the periventricular white matter (P < 0.001), and neuronal loss in the hippocampus and basal ganglia (P < 0.001), with suppression of activated caspase-3 and microglia (isolectin-B4 positive). Proliferation was significantly reduced in periventricular white matter after occlusion (P < 0.05), but not improved after hypothermia. In conclusion, delayed, prolonged head cooling after a profound hypoxic insult in the preterm fetus was associated with a significant reduction in loss of neurons and immature oligodendroglia, with evidence of EEG and haemodynamic improvement after 3 days recovery, but also with a persisting reduction in proliferation of cells in the periventricular region. Further studies are required to evaluate the long-term impact of cooling on brain growth and maturation.

Original languageEnglish
Pages (from-to)491-506
Number of pages16
JournalJournal of Physiology
Issue number2
Publication statusPublished - Jan 15 2007
Externally publishedYes

ASJC Scopus subject areas

  • Physiology


Dive into the research topics of 'The effect of cerebral hypothermia on white and grey matter injury induced by severe hypoxia in preterm fetal sheep'. Together they form a unique fingerprint.

Cite this