Abstract
The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 × 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 × 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.
Original language | English |
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Pages (from-to) | 1077-1085 |
Number of pages | 9 |
Journal | International Urogynecology Journal |
Volume | 18 |
Issue number | 9 |
DOIs | |
Publication status | Published - Sept 2007 |
Keywords
- Ageing
- Incontinence
- Menopause
- Rat
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Urology