The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats

Diaa E.E. Rizk; Mohamed A. Fahim; Hazem A. Hassan; Ahmed H. Al-Marzouqi; Gaber A. Ramadan; Soha S. Al-Kedrah; Layla S. Al-Ghafri

doi:10.1007/s00192-006-0278-4

The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats

Diaa E.E. Rizk, Mohamed A. Fahim, Hazem A. Hassan, Ahmed H. Al-Marzouqi, Gaber A. Ramadan, Soha S. Al-Kedrah, Layla S. Al-Ghafri

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 × 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 × 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27^kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.

Original language	English
Pages (from-to)	1077-1085
Number of pages	9
Journal	International Urogynecology Journal
Volume	18
Issue number	9
DOIs	https://doi.org/10.1007/s00192-006-0278-4
Publication status	Published - Sept 2007

Keywords

Ageing
Incontinence
Menopause
Rat

ASJC Scopus subject areas

Obstetrics and Gynaecology
Urology

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10.1007/s00192-006-0278-4

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title = "The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats",

abstract = "The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 × 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 × 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.",

keywords = "Ageing, Incontinence, Menopause, Rat",

author = "Rizk, {Diaa E.E.} and Fahim, {Mohamed A.} and Hassan, {Hazem A.} and Al-Marzouqi, {Ahmed H.} and Ramadan, {Gaber A.} and Al-Kedrah, {Soha S.} and Al-Ghafri, {Layla S.}",

note = "Funding Information: Acknowledgment This study was supported by a research grant from the Research Affairs, United Arab Emirates University, N 01-01-8-12/04. We would like to thank Mr. S. Daoud and Mr. M. Shafiullah, respectively, for technical assistance with processing of tissue samples and the ovariectomy procedure.",

year = "2007",

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doi = "10.1007/s00192-006-0278-4",

language = "English",

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AU - Rizk, Diaa E.E.

AU - Fahim, Mohamed A.

AU - Hassan, Hazem A.

AU - Al-Marzouqi, Ahmed H.

AU - Ramadan, Gaber A.

AU - Al-Kedrah, Soha S.

AU - Al-Ghafri, Layla S.

N1 - Funding Information: Acknowledgment This study was supported by a research grant from the Research Affairs, United Arab Emirates University, N 01-01-8-12/04. We would like to thank Mr. S. Daoud and Mr. M. Shafiullah, respectively, for technical assistance with processing of tissue samples and the ovariectomy procedure.

PY - 2007/9

Y1 - 2007/9

N2 - The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 × 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 × 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.

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KW - Incontinence

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The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats

Abstract

Keywords

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