The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats

Diaa E.E. Rizk; Mohamed A. Fahim; Hazem A. Hassan; Ahmed H. Al-Marzouqi; Gaber A. Ramadan; Soha S. Al-Kedrah; Layla S. Al-Ghafri

doi:10.1007/s00192-006-0278-4

The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats

Diaa E.E. Rizk, Mohamed A. Fahim, Hazem A. Hassan, Ahmed H. Al-Marzouqi, Gaber A. Ramadan, Soha S. Al-Kedrah, Layla S. Al-Ghafri

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 × 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 × 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27^kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.

Original language	English
Pages (from-to)	1077-1085
Number of pages	9
Journal	International Urogynecology Journal
Volume	18
Issue number	9
DOIs	https://doi.org/10.1007/s00192-006-0278-4
Publication status	Published - Sept 2007

Keywords

Ageing
Incontinence
Menopause
Rat

ASJC Scopus subject areas

Obstetrics and Gynaecology
Urology

Access to Document

10.1007/s00192-006-0278-4

Cite this

@article{d26a4c46329949febc3233b1308b9008,

title = "The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats",

abstract = "The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 × 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 × 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.",

keywords = "Ageing, Incontinence, Menopause, Rat",

author = "Rizk, {Diaa E.E.} and Fahim, {Mohamed A.} and Hassan, {Hazem A.} and Al-Marzouqi, {Ahmed H.} and Ramadan, {Gaber A.} and Al-Kedrah, {Soha S.} and Al-Ghafri, {Layla S.}",

note = "Funding Information: Acknowledgment This study was supported by a research grant from the Research Affairs, United Arab Emirates University, N 01-01-8-12/04. We would like to thank Mr. S. Daoud and Mr. M. Shafiullah, respectively, for technical assistance with processing of tissue samples and the ovariectomy procedure.",

year = "2007",

month = sep,

doi = "10.1007/s00192-006-0278-4",

language = "English",

volume = "18",

pages = "1077--1085",

journal = "International Urogynecology Journal",

issn = "0937-3462",

publisher = "Springer Nature",

number = "9",

}

TY - JOUR

T1 - The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats

AU - Rizk, Diaa E.E.

AU - Fahim, Mohamed A.

AU - Hassan, Hazem A.

AU - Al-Marzouqi, Ahmed H.

AU - Ramadan, Gaber A.

AU - Al-Kedrah, Soha S.

AU - Al-Ghafri, Layla S.

N1 - Funding Information: Acknowledgment This study was supported by a research grant from the Research Affairs, United Arab Emirates University, N 01-01-8-12/04. We would like to thank Mr. S. Daoud and Mr. M. Shafiullah, respectively, for technical assistance with processing of tissue samples and the ovariectomy procedure.

PY - 2007/9

Y1 - 2007/9

N2 - The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 × 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 × 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.

AB - The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 × 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 × 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.

KW - Ageing

KW - Incontinence

KW - Menopause

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=35748951324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35748951324&partnerID=8YFLogxK

U2 - 10.1007/s00192-006-0278-4

DO - 10.1007/s00192-006-0278-4

M3 - Article

C2 - 17205219

AN - SCOPUS:35748951324

SN - 0937-3462

VL - 18

SP - 1077

EP - 1085

JO - International Urogynecology Journal

JF - International Urogynecology Journal

IS - 9

ER -

The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this