The Effect of Oxytocin on Intracellular Ca2+ Release in Cardiac Cells

Anatoliy Shmygol, Sanaa H.S.H. Alameri, Khadeijah Y.K.M. Almemari, Naema M.A.J. Alsuwaidi, Ifrah I. Ali

Research output: Chapter in Book/Report/Conference proceedingChapter


Ca2+ signaling is vital for the proper functioning of all cells, including cells of the cardiovascular system. Membrane receptors for many hormones trigger intracellular Ca2+ signaling via the activation of phospholipase C and production of inositol-1,4,5-trisphosphate (InsP3). Several research groups have demonstrated the expression of oxytocin (OXT) and oxytocin receptors (OXTR) in the heart and suggested a cardioprotective role of OXT against several pathological conditions. Here we describe the protocol for measuring the effects of oxytocin on intracellular Ca2+ dynamics in newborn rat cardiac myocytes and cardiac fibroblasts maintained in short-term culture.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Ca signaling
  • Cal-520
  • Cardiac fibroblast
  • Cardiac myocyte
  • Endoplasmic reticulum
  • IP receptors
  • Oxytocin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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