TY - JOUR
T1 - The genome of pest Rhynchophorus ferrugineus reveals gene families important at the plant-beetle interface
AU - Hazzouri, Khaled Michel
AU - Sudalaimuthuasari, Naganeeswaran
AU - Kundu, Biduth
AU - Nelson, David
AU - Al-Deeb, Mohammad Ali
AU - Le Mansour, Alain
AU - Spencer, Johnston J.
AU - Desplan, Claude
AU - Amiri, Khaled M.A.
N1 - Funding Information:
We would like to thank all the personnel of the Khalifa center for Genetic Engineering and Biotechnology (KCGEB) who made this phase one of the project come true. A special thanks to Mr. Sajid at KCGEB for taking pictures of R. ferrugineus. This project was funded by the Khalifa Center for Genetic Engineering and Biotechnology (KCGEB) and by the New York University Abu Dhabi Research Institute (G-1205C and G-1205i).
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - The red palm weevil, Rhynchophorus ferrugineus, infests palm plantations, leading to large financial losses and soil erosion. Pest-host interactions are poorly understood in R. ferrugineus, but the analysis of genetic diversity and pest origins will help advance efforts to eradicate this pest. We sequenced the genome of R. ferrugineus using a combination of paired-end Illumina sequencing (150 bp), Oxford Nanopore long reads, 10X Genomics and synteny analysis to produce an assembly with a scaffold N50 of ~60 Mb. Structural variations showed duplication of detoxifying and insecticide resistance genes (e.g., glutathione S-transferase, P450, Rdl). Furthermore, the evolution of gene families identified those under positive selection including one glycosyl hydrolase (GH16) gene family, which appears to result from horizontal gene transfer. This genome will be a valuable resource to understand insect evolution and behavior and to allow the genetic modification of key genes that will help control this pest.
AB - The red palm weevil, Rhynchophorus ferrugineus, infests palm plantations, leading to large financial losses and soil erosion. Pest-host interactions are poorly understood in R. ferrugineus, but the analysis of genetic diversity and pest origins will help advance efforts to eradicate this pest. We sequenced the genome of R. ferrugineus using a combination of paired-end Illumina sequencing (150 bp), Oxford Nanopore long reads, 10X Genomics and synteny analysis to produce an assembly with a scaffold N50 of ~60 Mb. Structural variations showed duplication of detoxifying and insecticide resistance genes (e.g., glutathione S-transferase, P450, Rdl). Furthermore, the evolution of gene families identified those under positive selection including one glycosyl hydrolase (GH16) gene family, which appears to result from horizontal gene transfer. This genome will be a valuable resource to understand insect evolution and behavior and to allow the genetic modification of key genes that will help control this pest.
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U2 - 10.1038/s42003-020-1060-8
DO - 10.1038/s42003-020-1060-8
M3 - Article
C2 - 32581279
AN - SCOPUS:85087092872
SN - 2399-3642
VL - 3
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 323
ER -