The genome, pangenome, and physiological analysis of Leclercia adecarboxylata (kcgeb_e1), a plant growth-promoting bacterium

Esam Eldin Saeed, Naganeeswaran Sudalaimuthuasari, Fayas Thayale Purayil, Mohammed Rafi, Biduth Kundu, Ajay Kumar Mishra, Raja Saeed Al-Maskari, Amira Mohamed Abdelfattah, Afaf Kamal Eldin, Suja George, Miranda Procter, Khaled M. Hazzouri, Khaled M.A. Amiri

Research output: Contribution to journalArticlepeer-review

Abstract

Plant growth-promoting bacteria (PGPB) as biofertilizer plays an important role in agriculture practices. In this study, we isolated and identified plant-associated bacteria Leclercia adecarboxylata (kcgeb_e1) from the root region of the halophytic plant Sesuvium verrucosum. We tested its physiological activity and the effect of inoculation, with and without salt, on photosynthesis using Cajanus cajan. Further, we sequenced the whole genome of L. adecarboxylata (kcgeb_e1) and carried out pangenome analysis with 12 other genomes of the same species, which highlights unique genes enriched for pathways involved in abiotic stress tolerance (salinity, drought and heat) and carbohydrate transport. Moreover, gene families involved in abiotic stress tolerance, host adhesion, and transport were under positive selection (e.g., Aldo/keto reductase family, Hemagglutinin, Porin, and sugar transport). We observed a loss of ACC deaminase gene in this pangenome; however, this strain can still produce 1-aminocyclopropane-1-carboxylate (ACC), an enhancer of abiotic stress, which suggests that its homologue, d-cysteine sulfatase, has a bifunctional activity. In addition, this strain has Indole acetic acid (IAA) and phosphate solubilization activity. Combining these findings with the efficiency of colonizing the root surface of Solanum lycopersicum, this strain showed remarkable enhancement of photosynthesis, comparing control to inoculated plants. This increase in photosynthesis is consistent with an increase in sucrose under salt treatment, but not in glucose and fructose, which acts as a sensor in opposing the negative effect of salinity and promoting sustainable growth. Given all this, our study suggests that this PGPB can act as a biofertilizer for sustainable agriculture.

Original languageEnglish
Article number76
JournalDiscover Applied Sciences
Volume6
Issue number3
DOIs
Publication statusPublished - Mar 2024

Keywords

  • Abiotic stress
  • Biofertilizer
  • Indole acetic acid
  • Phosphate solubilization
  • Salt stress
  • Sesuvium verrucosum

ASJC Scopus subject areas

  • General Chemical Engineering
  • General Earth and Planetary Sciences
  • General Engineering
  • General Environmental Science
  • General Materials Science
  • General Physics and Astronomy

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