TY - JOUR
T1 - The K-segments of the wheat dehydrin DHN-5 are essential for the protection of lactate dehydrogenase and β-glucosidase activities in vitro
AU - Drira, Marwa
AU - Saibi, Walid
AU - Brini, Faïçal
AU - Gargouri, Ali
AU - Masmoudi, Khaled
AU - Hanin, Moez
N1 - Funding Information:
Acknowledgments This study was supported by grants from the Ministry of Higher Education and Scientific Research, Tunisia.
PY - 2013/6
Y1 - 2013/6
N2 - The wheat dehydrin DHN-5 has been previously shown to exhibit heat protecting effect on enzymatic activities. In order to understand the molecular mechanism by which DHN-5 exerts its protective function, we performed an approach to dissect the functional domains of DHN-5 responsible for this feature. In two distinct enzymatic assays, we found that the truncated forms of DHN-5 containing only one K- or two K-segments are able to protect albeit to less extent than the wild type protein, lactate dehydrogenase and β-glucosidase against damage induced by various stresses in vitro. However, the YS- and Φ-segments alone have no protective effects on these enzymes. Therefore, our study provides the evidence that the protective function of DHN-5 seems to be directly linked to its K-segments which through their amphipatic α-helical structure, may act to prevent protein aggregation.
AB - The wheat dehydrin DHN-5 has been previously shown to exhibit heat protecting effect on enzymatic activities. In order to understand the molecular mechanism by which DHN-5 exerts its protective function, we performed an approach to dissect the functional domains of DHN-5 responsible for this feature. In two distinct enzymatic assays, we found that the truncated forms of DHN-5 containing only one K- or two K-segments are able to protect albeit to less extent than the wild type protein, lactate dehydrogenase and β-glucosidase against damage induced by various stresses in vitro. However, the YS- and Φ-segments alone have no protective effects on these enzymes. Therefore, our study provides the evidence that the protective function of DHN-5 seems to be directly linked to its K-segments which through their amphipatic α-helical structure, may act to prevent protein aggregation.
KW - Abiotic stress
KW - Dehydrins
KW - K-segment
KW - Lactate dehydrogenase
KW - β-Glucosidase
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U2 - 10.1007/s12033-012-9606-8
DO - 10.1007/s12033-012-9606-8
M3 - Article
C2 - 23054631
AN - SCOPUS:84882879839
SN - 1073-6085
VL - 54
SP - 643
EP - 650
JO - Molecular Biotechnology
JF - Molecular Biotechnology
IS - 2
ER -