The origins of the sciatic nerve and changes in neuropeptides after axotomy: a double labelling study using retrograde transport of Tru blue and vasoactive intestinal polypeptide immunohistochemistry

S. A.S. Shehab; M. E. Atkinson; J. N. Payne

doi:10.1016/0006-8993(86)90914-5

The origins of the sciatic nerve and changes in neuropeptides after axotomy: a double labelling study using retrograde transport of Tru blue and vasoactive intestinal polypeptide immunohistochemistry

S. A.S. Shehab, M. E. Atkinson, J. N. Payne

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

One month after sciatic nerve section, only dorsal root ganglion cells which take up True blue applied to the cut end of the nerve show vasoactive intestinal polypeptide immunoreactivity. This indicates that VIP expression is only in cells with damaged axons. Motor axons with True blue-positive perikarya in the ventral horn originate from the fourth to sixth lumbar segments whereas unmyelinated and small myelinated sensory nerves terminate in the third to fifth lumbar segments of the spinal cord.

Original language	English
Pages (from-to)	180-185
Number of pages	6
Journal	Brain Research
Volume	376
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(86)90914-5
Publication status	Published - Jun 18 1986
Externally published	Yes

Keywords

dorsal root ganglion
fluorescence microscopy
immunohistochemistry
peripheral axotomy
retrograde transport
sciatic nerve
vasoactive intestinal polypeptide (VIP)

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(86)90914-5

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The origins of the sciatic nerve and changes in neuropeptides after axotomy: a double labelling study using retrograde transport of Tru blue and vasoactive intestinal polypeptide immunohistochemistry. / Shehab, S. A.S.; Atkinson, M. E.; Payne, J. N.
In: Brain Research, Vol. 376, No. 1, 18.06.1986, p. 180-185.

Research output: Contribution to journal › Article › peer-review

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abstract = "One month after sciatic nerve section, only dorsal root ganglion cells which take up True blue applied to the cut end of the nerve show vasoactive intestinal polypeptide immunoreactivity. This indicates that VIP expression is only in cells with damaged axons. Motor axons with True blue-positive perikarya in the ventral horn originate from the fourth to sixth lumbar segments whereas unmyelinated and small myelinated sensory nerves terminate in the third to fifth lumbar segments of the spinal cord.",

keywords = "dorsal root ganglion, fluorescence microscopy, immunohistochemistry, peripheral axotomy, retrograde transport, sciatic nerve, vasoactive intestinal polypeptide (VIP)",

author = "Shehab, {S. A.S.} and Atkinson, {M. E.} and Payne, {J. N.}",

note = "Funding Information: The authors are grateful to Ms. Maureen Tune for preparing photomicrographs. S.A.S.S. is supported by a scholarship from the University of Basrah, Republic of Iraq. Antibodies were purchased from a grant to M.E.A. from the Sheffield University Medical and Dental Research fund.",

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AU - Atkinson, M. E.

AU - Payne, J. N.

N1 - Funding Information: The authors are grateful to Ms. Maureen Tune for preparing photomicrographs. S.A.S.S. is supported by a scholarship from the University of Basrah, Republic of Iraq. Antibodies were purchased from a grant to M.E.A. from the Sheffield University Medical and Dental Research fund.

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AB - One month after sciatic nerve section, only dorsal root ganglion cells which take up True blue applied to the cut end of the nerve show vasoactive intestinal polypeptide immunoreactivity. This indicates that VIP expression is only in cells with damaged axons. Motor axons with True blue-positive perikarya in the ventral horn originate from the fourth to sixth lumbar segments whereas unmyelinated and small myelinated sensory nerves terminate in the third to fifth lumbar segments of the spinal cord.

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KW - fluorescence microscopy

KW - immunohistochemistry

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KW - retrograde transport

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KW - vasoactive intestinal polypeptide (VIP)

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The origins of the sciatic nerve and changes in neuropeptides after axotomy: a double labelling study using retrograde transport of Tru blue and vasoactive intestinal polypeptide immunohistochemistry

Abstract

Keywords

ASJC Scopus subject areas

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