The Role of Tenascin-C on the Structural Plasticity of Perineuronal Nets and Synaptic Expression in the Hippocampus of Male Mice

Ana Jakovljević, Vera Stamenković, Joko Poleksić, Mohammad I.K. Hamad, Gebhard Reiss, Igor Jakovcevski, Pavle R. Andjus

Research output: Contribution to journalArticlepeer-review

Abstract

Neuronal plasticity is a crucial mechanism for an adapting nervous system to change. It is shown to be regulated by perineuronal nets (PNNs), the condensed forms of the extracellular matrix (ECM) around neuronal bodies. By assessing the changes in the number, intensity, and structure of PNNs, the ultrastructure of the PNN mesh, and the expression of inhibitory and excitatory synaptic inputs on these neurons, we aimed to clarify the role of an ECM glycoprotein, tenascin-C (TnC), in the dorsal hippocampus. To enhance neuronal plasticity, TnC-deficient (TnC-/-) and wild-type (TnC+/+) young adult male mice were reared in an enriched environment (EE) for 8 weeks. Deletion of TnC in TnC-/- mice showed an ultrastructural reduction of the PNN mesh and an increased inhibitory input in the dentate gyrus (DG), and an increase in the number of PNNs with a rise in the inhibitory input in the CA2 region. EE induced an increased inhibitory input in the CA2, CA3, and DG regions; in DG, the change was also followed by an increased intensity of PNNs. No changes in PNNs or synaptic expression were found in the CA1 region. We conclude that the DG and CA2 regions emerged as focal points of alterations in PNNs and synaptogenesis with EE as mediated by TnC.

Original languageEnglish
Article number508
JournalBiomolecules
Volume14
Issue number4
DOIs
Publication statusPublished - Apr 2024

Keywords

  • enriched environment
  • hippocampus
  • perineuronal nets
  • synaptic plasticity
  • tenascin-C

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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