TY - JOUR
T1 - Tissue specific expression and immunohistochemical localization of glutathione S-transferase in streptozotocin induced diabetic rats
T2 - Modulation by Momordica charantia (karela) extract
AU - Raza, Haider
AU - Ahmed, Ijaz
AU - John, Annie
N1 - Funding Information:
The supports from UAE Terry Fox Cancer Research Fund and a Research Committee Grant from the Faculty of Medicine and Health Sciences (to H.R) are gratefully acknowledged. A part of this work has been presented for the partial fulfillment of the Ph.D. thesis for I. Ahmed from Lancaster University; U.K. Authors wish to thank Drs. E. Adeghate, and A. K. Sharma, members of the thesis advisory committee for their continuous support and encouragement in this work. Thanks are also due to Drs M.Gillett and Lekha A. Kumar for critical reading and Mr. C. Chathanath in preparation of this manuscript.
PY - 2004/2/6
Y1 - 2004/2/6
N2 - In streptozotocin (STZ)-induced diabetes, destruction of pancreatic beta-cell causes an acute shortage of insulin. Increased oxidative stress is believed to be one of the main factors in the etiology and complications of diabetes. In this study we have reported hyperglycemia and glutathione- associated oxidative stress in rats one week after treatment with STZ. In our previous studies, we have reported oxidative stress-related changes in xenobiotic metabolism in tissues from STZ-induced chronic diabetic rats. Here, we demonstrate by immunohistochemistry, that glutathione S-transferase (GST) isoenzymes are differentially expressed in the liver, kidney and testis of diabetic rats. The distribution of GST isoenzymes was found to be tissue- and regio-specific. In addition, we have also shown that treatment with an extract of Momordica charantia (karela), an antidiabetic herb, modulates GST expression in diabetic rats and reverts them to the normal distribution as seen in the tissues of control rats. These results suggest that glutathione metabolism and GST distribution in the tissues of diabetic rats may play an important role in the etiology, pathology and prevention of diabetes.
AB - In streptozotocin (STZ)-induced diabetes, destruction of pancreatic beta-cell causes an acute shortage of insulin. Increased oxidative stress is believed to be one of the main factors in the etiology and complications of diabetes. In this study we have reported hyperglycemia and glutathione- associated oxidative stress in rats one week after treatment with STZ. In our previous studies, we have reported oxidative stress-related changes in xenobiotic metabolism in tissues from STZ-induced chronic diabetic rats. Here, we demonstrate by immunohistochemistry, that glutathione S-transferase (GST) isoenzymes are differentially expressed in the liver, kidney and testis of diabetic rats. The distribution of GST isoenzymes was found to be tissue- and regio-specific. In addition, we have also shown that treatment with an extract of Momordica charantia (karela), an antidiabetic herb, modulates GST expression in diabetic rats and reverts them to the normal distribution as seen in the tissues of control rats. These results suggest that glutathione metabolism and GST distribution in the tissues of diabetic rats may play an important role in the etiology, pathology and prevention of diabetes.
KW - Diabetes
KW - Glutathione S-transferase
KW - Immunohistochemistry
KW - Karela
KW - Oxidative stress
KW - Streptozotocin
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U2 - 10.1016/j.lfs.2003.08.023
DO - 10.1016/j.lfs.2003.08.023
M3 - Article
C2 - 14729399
AN - SCOPUS:0346654087
SN - 0024-3205
VL - 74
SP - 1503
EP - 1511
JO - Life Sciences
JF - Life Sciences
IS - 12
ER -