Toxins from the venom of the green mamba Dendroaspis angusticeps that inhibit the binding of quinuclidinyl benzilate to muscarinic acetylcholine receptors

Abdu Adem; Anette Åsblom; Gunnar Johansson; Paul M. Mbugua; Evert Karlsson

doi:10.1016/0167-4889(88)90025-0

Toxins from the venom of the green mamba Dendroaspis angusticeps that inhibit the binding of quinuclidinyl benzilate to muscarinic acetylcholine receptors

Abdu Adem, Anette Åsblom, Gunnar Johansson, Paul M. Mbugua, Evert Karlsson

Research output: Contribution to journal › Article › peer-review

90 Citations (Scopus)

Abstract

Two protein toxins that displace the muscarinic antagonist quinuclidinyl benzilate from rat cortex synaptosomal membranes have been isolated from the green mamba (Dendroaspis angusticeps) venom by gel filtration on sephadex G-50, chromatography on the ion-exchangers Bio-Rex 70 and Sulphopropyl-Sephadex C-25 and reversed-phase HPLC. Toxin 1 has 64 amino acids and four disulfides and a formula weight of 7200 and the corresponding values for toxin 2 are 63, 4 and 6840, respectively. Ultracentrifugation gave a molecular weight of 6900 for toxin 1 and 6700 for toxin 2, Quinuclidinyl benzilate that binds to all types of muscarinic cholinergic receptor was displaced to about 50% by both toxins. This partial displacement indicates that the toxins might be specific for one subtype of receptor.

Original language	English
Pages (from-to)	340-345
Number of pages	6
Journal	BBA - Molecular Cell Research
Volume	968
Issue number	3
DOIs	https://doi.org/10.1016/0167-4889(88)90025-0
Publication status	Published - Mar 11 1988
Externally published	Yes

Keywords

(Green mamba)
Ligand binding
Muscarinic cholinergic receptor
Quinuclidinyl benzilate
Snake toxin

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/0167-4889(88)90025-0

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title = "Toxins from the venom of the green mamba Dendroaspis angusticeps that inhibit the binding of quinuclidinyl benzilate to muscarinic acetylcholine receptors",

abstract = "Two protein toxins that displace the muscarinic antagonist quinuclidinyl benzilate from rat cortex synaptosomal membranes have been isolated from the green mamba (Dendroaspis angusticeps) venom by gel filtration on sephadex G-50, chromatography on the ion-exchangers Bio-Rex 70 and Sulphopropyl-Sephadex C-25 and reversed-phase HPLC. Toxin 1 has 64 amino acids and four disulfides and a formula weight of 7200 and the corresponding values for toxin 2 are 63, 4 and 6840, respectively. Ultracentrifugation gave a molecular weight of 6900 for toxin 1 and 6700 for toxin 2, Quinuclidinyl benzilate that binds to all types of muscarinic cholinergic receptor was displaced to about 50% by both toxins. This partial displacement indicates that the toxins might be specific for one subtype of receptor.",

keywords = "(Green mamba), Ligand binding, Muscarinic cholinergic receptor, Quinuclidinyl benzilate, Snake toxin",

author = "Abdu Adem and Anette {\AA}sblom and Gunnar Johansson and Mbugua, {Paul M.} and Evert Karlsson",

note = "Funding Information: This work was supported by grants from the Swedish Natural Science Research Council, and O.E. and Edla Johansson's Science Foundation.",

year = "1988",

month = mar,

day = "11",

doi = "10.1016/0167-4889(88)90025-0",

language = "English",

volume = "968",

pages = "340--345",

journal = "BBA - Molecular Cell Research",

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T1 - Toxins from the venom of the green mamba Dendroaspis angusticeps that inhibit the binding of quinuclidinyl benzilate to muscarinic acetylcholine receptors

AU - Adem, Abdu

AU - Åsblom, Anette

AU - Johansson, Gunnar

AU - Mbugua, Paul M.

AU - Karlsson, Evert

N1 - Funding Information: This work was supported by grants from the Swedish Natural Science Research Council, and O.E. and Edla Johansson's Science Foundation.

PY - 1988/3/11

Y1 - 1988/3/11

N2 - Two protein toxins that displace the muscarinic antagonist quinuclidinyl benzilate from rat cortex synaptosomal membranes have been isolated from the green mamba (Dendroaspis angusticeps) venom by gel filtration on sephadex G-50, chromatography on the ion-exchangers Bio-Rex 70 and Sulphopropyl-Sephadex C-25 and reversed-phase HPLC. Toxin 1 has 64 amino acids and four disulfides and a formula weight of 7200 and the corresponding values for toxin 2 are 63, 4 and 6840, respectively. Ultracentrifugation gave a molecular weight of 6900 for toxin 1 and 6700 for toxin 2, Quinuclidinyl benzilate that binds to all types of muscarinic cholinergic receptor was displaced to about 50% by both toxins. This partial displacement indicates that the toxins might be specific for one subtype of receptor.

AB - Two protein toxins that displace the muscarinic antagonist quinuclidinyl benzilate from rat cortex synaptosomal membranes have been isolated from the green mamba (Dendroaspis angusticeps) venom by gel filtration on sephadex G-50, chromatography on the ion-exchangers Bio-Rex 70 and Sulphopropyl-Sephadex C-25 and reversed-phase HPLC. Toxin 1 has 64 amino acids and four disulfides and a formula weight of 7200 and the corresponding values for toxin 2 are 63, 4 and 6840, respectively. Ultracentrifugation gave a molecular weight of 6900 for toxin 1 and 6700 for toxin 2, Quinuclidinyl benzilate that binds to all types of muscarinic cholinergic receptor was displaced to about 50% by both toxins. This partial displacement indicates that the toxins might be specific for one subtype of receptor.

KW - (Green mamba)

KW - Ligand binding

KW - Muscarinic cholinergic receptor

KW - Quinuclidinyl benzilate

KW - Snake toxin

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Toxins from the venom of the green mamba Dendroaspis angusticeps that inhibit the binding of quinuclidinyl benzilate to muscarinic acetylcholine receptors

Abstract

Keywords

ASJC Scopus subject areas

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