Transformation of two potatocultivars 'Spunta' and 'Claustar' (Solanum tuberosum) with lettuce mosaic virus coat protein gene and heterologous immunity to potato virus Y

Afif Hassairi, Khaled Masmoudi, Josette Albouy, Christophe Robaglia, Marc Jullien, Radhouane Ellouz

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

The coat protein (CP) gene of the lettuce mosaic potyvirus (LMV) was introduced into two potato cultivars - Spunta and Claustar - via Agrobacterium tumefaciens-mediated transformation. Gene expression was verified by Southern, Northern and Western blot. The regenerated plants have been tested for resistance against potato virus Y (PVY-0) in greenhouse. We observed an immune phenotype in four transgenic lines expressing the CP-LMV protein at different detectable levels. However, in two other transgenic plant lines, symptoms were developed at a frequency of 25 and 60% with a delay in their appearance. Transgene transcripts of the CP-LMV gene were detected by Northern blot in these two lines, but the protein could not be detected by Western blot. The correlation between the presence of the CP protein at a detectable level and the expression of an immune phenotype is discussed.

Original languageEnglish
Pages (from-to)31-42
Number of pages12
JournalPlant Science
Volume136
Issue number1
DOIs
Publication statusPublished - Aug 7 1998
Externally publishedYes

Keywords

  • Heterologous-resistance
  • LMV
  • PVY
  • Transgenic potato
  • Virus resistance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Fingerprint

Dive into the research topics of 'Transformation of two potatocultivars 'Spunta' and 'Claustar' (Solanum tuberosum) with lettuce mosaic virus coat protein gene and heterologous immunity to potato virus Y'. Together they form a unique fingerprint.

Cite this