TY - JOUR
T1 - Transport of Metanil Yellow in the Rat Plasma and Interaction of Its Metabolite, p-Aminodiphenylamine with Serum Proteins
AU - Raza, H.
AU - Khanna, S. K.
AU - Singh, G. B.
AU - Krishna Murti, C. R.
N1 - Funding Information:
One of us (HR) is thankful to the Council of Scientific and Industrial Research, New Delhi for award of Senior Research Fellowship. Thanks are also due to Messrs. P. S. Shukla and S. H. Mehdi for typography and photography, respectively.
PY - 1983/7/1
Y1 - 1983/7/1
N2 - Binding affinity of metanil yellow and its breakdown product p-aminodiphenylamine to serum proteins has been studied employing chromatographic separation on Sephadex G-200 and by paper and polyacrylamide gel electrophoresis. Metanil yellow has more affinity towards albumin than to globulins. The complexing is presumably through electrostatic forces. p-Aminodiphenylamine on the other hand, preferably binds to globulin fractions of serum protein. However, a stable binding with BSA alone was also observed. The binding was quite stable and was accompanied by a shift in absorbance from 430 nm to 500 nm. Aspartic acid moiety of protein was found to be one of the units involved in the binding of p-ADPA to proteins.
AB - Binding affinity of metanil yellow and its breakdown product p-aminodiphenylamine to serum proteins has been studied employing chromatographic separation on Sephadex G-200 and by paper and polyacrylamide gel electrophoresis. Metanil yellow has more affinity towards albumin than to globulins. The complexing is presumably through electrostatic forces. p-Aminodiphenylamine on the other hand, preferably binds to globulin fractions of serum protein. However, a stable binding with BSA alone was also observed. The binding was quite stable and was accompanied by a shift in absorbance from 430 nm to 500 nm. Aspartic acid moiety of protein was found to be one of the units involved in the binding of p-ADPA to proteins.
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U2 - 10.1080/02772248309357003
DO - 10.1080/02772248309357003
M3 - Article
AN - SCOPUS:0020959607
SN - 0277-2248
VL - 6
SP - 179
EP - 189
JO - Toxicological & Environmental Chemistry
JF - Toxicological & Environmental Chemistry
IS - 3
ER -