TY - JOUR
T1 - Two Novel Plant-Growth-Promoting Lelliottia amnigena Isolates from Euphorbia prostrata Aiton Enhance the Overall Productivity of Wheat and Tomato
AU - Parashar, Manisha
AU - Dhar, Sanjoy Kumar
AU - Kaur, Jaspreet
AU - Chauhan, Arjun
AU - Tamang, Jeewan
AU - Singh, Gajendra Bahadur
AU - Lyudmila, Asyakina
AU - Perveen, Kahkashan
AU - Khan, Faheema
AU - Bukhari, Najat A.
AU - Mudgal, Gaurav
AU - Gururani, Mayank Anand
N1 - Funding Information:
The authors would like to acknowledge the support provided by Researchers Supporting Project Number RSP2023R358, King Saud University, Riyadh, Saudi Arabia. The APC was funded by a UPAR grant [12S114] and a UAEU-AUA grant [12R172] provided to MAG by UAE University.
Funding Information:
The authors would like to acknowledge the support provided by Researchers Supporting Project Number RSP2023R358, King Saud University, Riyadh, Saudi Arabia. The authors also thank the UIBT, Chandigarh University, for infrastructural support and UCRD for registering MP and SKD as pre-doctoral students under the able supervision of GM. A special vote of thanks to JK for extending her valuable academic and research support to new Ph.D. entrants and for developing and nurturing a sound R&D theme over resilient plants’ translational biotechnology at research lab II in UIBT. Thanks as well to AL from Kemerovo State University (Kemerovo, Russia) and MAG from United Arab Emirates University (Al Ain, UAE), for extending their continued collaboration and support in instilling the in-house ‘Exploring Resilient Life” research initiative at UIBT, Chandigarh University. A special thanks as well to Priestly Shan Boaz, (Pro-VC, CU) for team leadership, moral support, and the additional workspace at the Kalpana Chawla Center (KCC), CU. Further, the works in the manuscript could not have been realized successfully without the services of the laboratory support staff who are equally to be thanked. The institutes, however, had no role in shaping the manuscript and/or in the decision to publish it. We dedicate our work to late Kiran K. Sharma (Executive Director, TERI, New Delhi, India; formerly a Principal Scientist at ICRISAT-CGIAR, Hyderabad, India), a research-career mentor for the corresponding authors (in this manuscript), who recently left for a heavenly abode, leaving with us a plethora of brainstormed plans for translational research in crop biotechnology.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/9
Y1 - 2023/9
N2 - Euphorbiaceae is a highly diverse family of plants ranging from trees to ground-dwelling minute plants. Many of these have multi-faceted attributes like ornamental, medicinal, industrial, and food-relevant values. In addition, they have been regarded as keystone resources for investigating plant-specific resilience mechanisms that grant them the dexterity to withstand harsh climates. In the present study, we isolated two co-culturable bacterial endophytes, EP1-AS and EP1-BM, from the stem internodal segments of the prostate spurge, Euphorbia prostrata, a plant member of the succulent family Euphorbiaceae. We characterized them using morphological, biochemical, and molecular techniques which revealed them as novel strains of Enterobacteriaceae, Lelliotia amnigena. Both the isolates significantly were qualified during the assaying of their plant growth promotion potentials. BM formed fast-growing swarms while AS showed growth as rounded colonies over nutrient agar. We validated the PGP effects of AS and BM isolates through in vitro and ex vitro seed-priming treatments with wheat and tomato, both of which resulted in significantly enhanced seed germination and morphometric and physiological plant growth profiles. In extended field trials, both AS and BM could remarkably also exhibit productive yields in wheat grain and tomato fruit harvests. This is probably the first-ever study in the context of PGPB endophytes in Euphorbia prostrata. We discuss our results in the context of promising agribiotechnology translations of the endophyte community associated with the otherwise neglected ground-dwelling spurges of Euphorbiaceae.
AB - Euphorbiaceae is a highly diverse family of plants ranging from trees to ground-dwelling minute plants. Many of these have multi-faceted attributes like ornamental, medicinal, industrial, and food-relevant values. In addition, they have been regarded as keystone resources for investigating plant-specific resilience mechanisms that grant them the dexterity to withstand harsh climates. In the present study, we isolated two co-culturable bacterial endophytes, EP1-AS and EP1-BM, from the stem internodal segments of the prostate spurge, Euphorbia prostrata, a plant member of the succulent family Euphorbiaceae. We characterized them using morphological, biochemical, and molecular techniques which revealed them as novel strains of Enterobacteriaceae, Lelliotia amnigena. Both the isolates significantly were qualified during the assaying of their plant growth promotion potentials. BM formed fast-growing swarms while AS showed growth as rounded colonies over nutrient agar. We validated the PGP effects of AS and BM isolates through in vitro and ex vitro seed-priming treatments with wheat and tomato, both of which resulted in significantly enhanced seed germination and morphometric and physiological plant growth profiles. In extended field trials, both AS and BM could remarkably also exhibit productive yields in wheat grain and tomato fruit harvests. This is probably the first-ever study in the context of PGPB endophytes in Euphorbia prostrata. We discuss our results in the context of promising agribiotechnology translations of the endophyte community associated with the otherwise neglected ground-dwelling spurges of Euphorbiaceae.
KW - Euphorbiaceae
KW - biofertilizer
KW - endophyte
KW - latex
KW - plant growth promotion
KW - productivity
KW - spurge
KW - succulent
KW - tomato
KW - wheat
UR - http://www.scopus.com/inward/record.url?scp=85170373247&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85170373247&partnerID=8YFLogxK
U2 - 10.3390/plants12173081
DO - 10.3390/plants12173081
M3 - Article
AN - SCOPUS:85170373247
SN - 2223-7747
VL - 12
JO - Plants
JF - Plants
IS - 17
M1 - 3081
ER -