Vaccination of calves with Mycobacteria bovis Bacilli Calmete Guerin (BCG) induced rapid increase in the proportion of peripheral blood γδ T cells

Joram Buza, Tadele Kiros, Adama Zerihun, Isaac Abraham, Gobena Ameni

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Changes in the proportion of peripheral blood T cell subsets after subcutaneous inoculation of cattle with Mycobacterium bovis Bacille Calmette-Guerin (BCG) were studied. Calves were injected with approximately 8 × 106 BCG bacillus and blood samples collected at weekly intervals for flow-cytometric analyses to determine the proportion of CD4+, CD8+ and γδ T cells. In addition, whole blood samples were stimulated in vitro with M. bovis purified protein derivative (PPD) and the secreted IFN-γ quantified by ELISA. Results showed cellular and cytokine changes which could be categorized into three phases. The first phase occurred within the first 2 weeks after vaccination involving an increase in proportion of WC1+ γδ T cells and a concomitant increase in the secretion of IFN-γ. These two responses peaked at 2 weeks and waned thereafter. The second phase involved an increase in the CD4/CD8 ratio as a result of an increase in the proportion of CD4+ T cells between 4 and 6 weeks. The third phase involved a decrease in the CD4/CD8 ratio due to an increase in the proportion of CD8+ T cells between 8 and 10 weeks. Surprisingly, the IFN-γ response was associated with changes in the γδ rather than the CD4+ or CD8+ T cells, suggesting that this cytokine was secreted by γδ-T cells. These results are consistent with the reported ability of γδ T cells to act rapidly and bridging the innate and classically adaptive immune responses.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalVeterinary Immunology and Immunopathology
Volume130
Issue number3-4
DOIs
Publication statusPublished - Aug 15 2009
Externally publishedYes

Keywords

  • BCG
  • CD4
  • CD8
  • Cattle
  • Mycobacterium bovis
  • T cells
  • Tuberculosis
  • γδ T cells

ASJC Scopus subject areas

  • Immunology
  • General Veterinary

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