@article{4da2b9579b6a4d0da85d597e6712d9fc,
title = "Validation of electrochemiluminescence assays for highly sensitive and reproducible quantification of a-synuclein in cerebrospinal fluid",
abstract = "α-Synuclein (aSyn), a putative cerebrospinal fluid biomarker, may support the diagnosis of neurodegenerative diseases. Previous studies led to conflicting results due to different preanalytical and analytical procedures. Standardized assays are required to allow for comparison of results from different laboratories. Materials & methods: We performed a side-by-side validation of a commercially available (Meso Scale Discovery, MD, USA) and a 'homebrew' assay for quantification of aSyn according to published guidelines. Results: The data showed high sensitivity and reproducibility for both assays. Preanalytical and analytical parameters did not affect the outcome of measurements. Conclusion: We conclude that both assays are very close in performance and suitable for research application of cerebrospinal fluid aSyn.",
keywords = "Assay, Electrochemiluminescence, Validation, α-synuclein",
author = "Niels Kruse and El-Agnaf, {Omar M.} and Brit Mollenhauer",
note = "Funding Information: This work was supported by the Michael J Fox Foundation for Parkinson's Research within the a-synuclein Assay Standardization LEAPS program (grant number 9977; www.michaeljfox.org/ foundation/grant-detail.php?grant-id=1427). B Mollenhauer has received independent research grants from TEVA-Pharma, Desitin, Boehringer Ingelheim, GE Healthcare and honoraria for consultancy from Bayer Schering Pharma AG, Roche, AbbVie, TEVA-Pharma, Biogen and for presentations from GlaxoSmithKline, Orion Pharma, TEVA-Pharma and travel costs from TEVA-Pharma. B Mollenhauer is a member of the executive steering committee of the Parkinson Progression Marker Initiative and the Systemic Synuclein Sampling Study of the Michael J Fox Foundation for Parkinson's Research and has received grants from the BMBF, EU, Deutsche Parkinson Vereinigung, Michael J Fox Foundation for Parkinson's Research, Stifterverband f{\"u}r die deutsche Wissenschaft, and has scientific collaborations with Roche, BM Squibb, E Lilly, Covance and Biogen. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Publisher Copyright: {\textcopyright} 2017 Future Science Ltd.",
year = "2017",
month = apr,
doi = "10.4155/bio-2017-0005",
language = "English",
volume = "9",
pages = "621--630",
journal = "Bioanalysis",
issn = "1757-6180",
publisher = "Future Science",
number = "8",
}