Vitamin A deficiency in mice enhances the colonic level of purine enzyme activity

Farida M. Al-Awadi, Sherif M. Karam, Islam Khan

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Background: Vitamin A is an important nutritional factor that regulates normal growth and functions of epithelial cells of the gastrointestinal tract. Objective: The objective of this study was to examine the role of vitamin A on the histological and biochemical changes in the colon of mice. Methods: To address this issue, vitamin A deficiency (VAD) was developed in mice by placing them on a VAD diet from weaning up to 120-170 days. Infiltration of inflammatory cells in the colon was determined histologically. Activities of adenosine deaminase, adenylate deaminase, purine nucleoside phosphorylase and myeloperoxidase were determined. Results: VAD in mice induced a significant increase in the number of mast cells per 100 crypts. There was also an abundance of other connective tissue cells such as plasma cells, lymphocytes and neutrophils around the crypts in the lamina propria. The colonic activity of adenosine deaminase and adenylate deaminase was increased due to VAD, whereas purine nucleoside phosphorylase activity remained unchanged. Immunohistochemical analysis showed an increased expression of adenosine deaminase in VAD mice colon. The increase in myeloperoxidase activity was not statistically significant. Conclusions: VAD causes upregulation of purine enzyme, which together with an increased number of inflammatory cells might exacerbate colonic injuries in VAD condition. (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalAnnals of Nutrition and Metabolism
Issue number4
Publication statusPublished - 2000
Externally publishedYes


  • Adenosine deaminase
  • Adenylate deaminase
  • Inflammatory cells
  • Myeloperoxidase
  • Vitamin A deficiency

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


Dive into the research topics of 'Vitamin A deficiency in mice enhances the colonic level of purine enzyme activity'. Together they form a unique fingerprint.

Cite this