Volatile general anaesthetic actions on recombinant nACh(α7), 5-HT3 and chimeric nACh(α7)-5-HT3 receptors expressed in Xenopus oocytes

Li Zhang; Murat Oz; Randall R. Stewart; Robert W. Peoples; Forrest F. Weight

doi:10.1038/sj.bjp.0700934

Volatile general anaesthetic actions on recombinant nACh(α7), 5-HT₃ and chimeric nACh(α7)-5-HT₃ receptors expressed in Xenopus oocytes

Li Zhang, Murat Oz, Randall R. Stewart, Robert W. Peoples, Forrest F. Weight

Research output: Contribution to journal › Article › peer-review

64 Citations (Scopus)

Abstract

The effect of halothane and isoflurane was studied on the function of recombinant neurotransmitter receptors expressed in Xenopus oocytes. Both anaesthetics inhibited nicotinic acetylcholine type α7 (nACh(α7)) receptor-mediated responses, potentiated 5-hydroxytryptamine type 3 (5-HT₃) receptor-mediated responses at low agonist concentrations, and inhibited the function of a chimeric receptor (with the N-terminal domain from the nACh(α7) receptor and the transmembrane and C-terminal domains from the 5-HT₃ receptor) in a manner similar to that of the nACh(α7) receptor. Since the N-terminal domain of the chimeric receptor was from the nACh(α7) receptor, the observations suggest that the inhibition involves the N-terminal domain of the receptor.

Original language	English
Pages (from-to)	353-355
Number of pages	3
Journal	British Journal of Pharmacology
Volume	120
Issue number	3
DOIs	https://doi.org/10.1038/sj.bjp.0700934
Publication status	Published - 1997
Externally published	Yes

Keywords

5-hydroxytryptamine receptor
Acetylcholine receptor
Anaesthetics
Ion channels
Molecular chimera
Receptors

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1038/sj.bjp.0700934

Cite this

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title = "Volatile general anaesthetic actions on recombinant nACh(α7), 5-HT3 and chimeric nACh(α7)-5-HT3 receptors expressed in Xenopus oocytes",

abstract = "The effect of halothane and isoflurane was studied on the function of recombinant neurotransmitter receptors expressed in Xenopus oocytes. Both anaesthetics inhibited nicotinic acetylcholine type α7 (nACh(α7)) receptor-mediated responses, potentiated 5-hydroxytryptamine type 3 (5-HT3) receptor-mediated responses at low agonist concentrations, and inhibited the function of a chimeric receptor (with the N-terminal domain from the nACh(α7) receptor and the transmembrane and C-terminal domains from the 5-HT3 receptor) in a manner similar to that of the nACh(α7) receptor. Since the N-terminal domain of the chimeric receptor was from the nACh(α7) receptor, the observations suggest that the inhibition involves the N-terminal domain of the receptor.",

keywords = "5-hydroxytryptamine receptor, Acetylcholine receptor, Anaesthetics, Ion channels, Molecular chimera, Receptors",

author = "Li Zhang and Murat Oz and Stewart, {Randall R.} and Peoples, {Robert W.} and Weight, {Forrest F.}",

year = "1997",

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AU - Oz, Murat

AU - Stewart, Randall R.

AU - Peoples, Robert W.

AU - Weight, Forrest F.

PY - 1997

Y1 - 1997

N2 - The effect of halothane and isoflurane was studied on the function of recombinant neurotransmitter receptors expressed in Xenopus oocytes. Both anaesthetics inhibited nicotinic acetylcholine type α7 (nACh(α7)) receptor-mediated responses, potentiated 5-hydroxytryptamine type 3 (5-HT3) receptor-mediated responses at low agonist concentrations, and inhibited the function of a chimeric receptor (with the N-terminal domain from the nACh(α7) receptor and the transmembrane and C-terminal domains from the 5-HT3 receptor) in a manner similar to that of the nACh(α7) receptor. Since the N-terminal domain of the chimeric receptor was from the nACh(α7) receptor, the observations suggest that the inhibition involves the N-terminal domain of the receptor.

AB - The effect of halothane and isoflurane was studied on the function of recombinant neurotransmitter receptors expressed in Xenopus oocytes. Both anaesthetics inhibited nicotinic acetylcholine type α7 (nACh(α7)) receptor-mediated responses, potentiated 5-hydroxytryptamine type 3 (5-HT3) receptor-mediated responses at low agonist concentrations, and inhibited the function of a chimeric receptor (with the N-terminal domain from the nACh(α7) receptor and the transmembrane and C-terminal domains from the 5-HT3 receptor) in a manner similar to that of the nACh(α7) receptor. Since the N-terminal domain of the chimeric receptor was from the nACh(α7) receptor, the observations suggest that the inhibition involves the N-terminal domain of the receptor.

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KW - Ion channels

KW - Molecular chimera

KW - Receptors

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