Vm-related extracellular potentials observed in red blood cells

Michael Pycraft Hughes, Emily J. Kruchek, Andrew D. Beale, Stephen J. Kitcatt, Sara Qureshi, Zachary P. Trott, Oriane Charbonnel, Paul A. Agbaje, Erin A. Henslee, Robert A. Dorey, Rebecca Lewis, Fatima H. Labeed

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Even in nonexcitable cells, the membrane potential Vm is fundamental to cell function, with roles from ion channel regulation, development, to cancer metastasis. Vm arises from transmembrane ion concentration gradients; standard models assume homogeneous extracellular and intracellular ion concentrations, and that Vm only exists across the cell membrane and has no significance beyond it. Using red blood cells, we show that this is incorrect, or at least incomplete; Vm is detectable beyond the cell surface, and modulating Vm produces quantifiable and consistent changes in extracellular potential. Evidence strongly suggests this is due to capacitive coupling between Vm and the electrical double layer, rather than molecular transporters. We show that modulating Vm changes the extracellular ion composition, mimicking the behaviour if voltage-gated ion channels in non-excitable channels. We also observed Vm-synchronised circadian rhythms in extracellular potential, with significant implications for cell–cell interactions and cardiovascular disease.

Original languageEnglish
Article number19446
JournalScientific reports
Volume11
Issue number1
DOIs
Publication statusPublished - Dec 2021
Externally publishedYes

ASJC Scopus subject areas

  • General

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