Waterpipe Smoke Inhalation Induces Lung Injury and Aortic Endothelial Dysfunction in Mice

Abderrahim Nemmar, Sumaya Beegam, Nur Elena Zaaba, Ozaz Elzaki, Afrin Pathan, Badreldin H. Ali

Research output: Contribution to journalArticlepeer-review


Summary Waterpipe tobacco smoking (WPS) inhalation has been shown to trigger endothelial dysfunction and atherosclerosis. However, the mechanisms underlying these effects are still unknown. Here, we assessed the impact and underlying mechanism of WPS exposure for one month on endothelial dysfunction using aortic tissue of mice. The duration of the session was 30 min/day and 5 days/week. Control mice were exposed to air. Inhalation of WPS induced an increase in the number of macrophages and neutrophils and the concentrations of protein, tumor necrosis factor α (TNF α), interleukin (IL)-1β, and glutathione in bronchoalveolar lavage fluid. Moreover, the concentrations of proinflammatory cytokines (TNF α, IL-6 and IL-1β), adhesion molecules (intercellular adhesion molecule-1, vascular cell adhesion molecule-1, E-selectin and Pselectin) and markers of oxidative stress (lipid peroxidation, glutathione, superoxide dismutase and nitric oxide) in aortic homogenates of mice exposed to WPS were significantly augmented compared with air-exposed mice. Likewise, the concentration of galectin-3 was significantly increased in the aortic homogenates of mice exposed to WPS compared with control group. WPS inhalation induced vascular DNA damage assessed by comet assay and apoptosis characterized by a significant increase in cleaved caspase-3. While the aortic expression of phosphorylated nuclear factor kB (NF-kB) was significantly increased following WPS inhalation, the concentration of sirtuin 1 (SIRT1) was significantly decreased in WPS group compared with air-exposed group. In conclusion, our study provided evidence that WPS inhalation triggers lung injury and endothelial inflammation, oxidative stress and apoptosis which were associated with nuclear factor-KB activation and SIRT1 down-regulation.

Original languageEnglish
Pages (from-to)337-347
Number of pages11
JournalPhysiological Research
Issue number3
Publication statusPublished - 2023


  • Aorta
  • Apoptosis
  • Inflammation
  • Oxidative stress
  • Waterpipe smoking

ASJC Scopus subject areas

  • Physiology


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