Wheat dehydrin DHN-5 exerts a heat-protective effect on β-glucosidase and glucose oxidase activities

Faiçal Brini, Walid Saibi, Imen Amara, Ali Gargouri, Khaled Masmoudi, Moez Hanin

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)


Group-2 late embryogenesis abundant (LEA) proteins, also known as dehydrins, are claimed to stabilize macromolecules against damage caused by freezing, dehydration, ionic or osmotic stresses. However, their precise function remains unknown. Here, we investigated the effect of wheat dehydrin (DHN-5) protein on the activity and thermostability of two distinct enzymes, β-glucosidase (bglG) and glucose oxidase/peroxidase (GOD/POD) in vitro. The purified DHN-5 protein had the capacity to preserve and stabilize the activity of bglG subjected to heat treatment. In addition, DHN-5 stabilized oxidizing enzymes, as it improved reliability in measuring glucose concentrations with a glucose oxidase/peroxidase (GOD/POD) kit while the temperature increased from 37 to 70 °C. All together the data presented provide evidence that DHN-5 is a dehydrin able to preserve enzyme activities in vitro from adverse effects induced by heating.

Original languageEnglish
Pages (from-to)1050-1054
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Issue number5
Publication statusPublished - 2010
Externally publishedYes


  • Dehydrin
  • Enzymatic activity
  • Glucose oxidase/peroxidase
  • Heating stress
  • β-Glucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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